Enteral nutrition and prevention of anastomotic leakage: A possible role for conditional essential amino acids? Results of a randomized clinical trialVan Barneveld, K W YBoelens, P GHeesakkers, F.F.B.MLuyer, M DBakker, J ARutten, H J T...
All primary porcine fibroblasts were cultured in high-glucose DMEM (HyClone) supplemented with 15% fetal bovine serum (Gibco), 1× Non-Essential Amino Acids (Gibco), 1× GlutaMax (Gibco), 1× sodium pyruvate (Gibco), and 1× penicillin-streptomycin (Gibco) and maintained in a cell ...
E14Tg2a embryonic stem cells were maintained in Dulbecco’s modified Eagle’s Medium (DMEM) supplemented with 15% fetal calf serum (FCS), 2 mM L-glutamine, 1 mM sodium pyruvate, 1% penicillin/streptomycin, 100 μM non-essential amino acids (all from Invitrogen), 100 μM β-merca...
The microbial cell wall is essential for maintenance of cell shape and resistance to external stressors1. The primary structural component of the cell wall is peptidoglycan, a glycopolymer with peptide crosslinks located outside of the cell membrane1. Peptidoglycan biosynthesis and structure are respons...
These results support our previous proposal of a novel mechanism linking carbon and nitrogen assimilation and relates IIANtr to the unknown process regulated by the essential GTPase Era.Previous article in issue Next article in issue Sorry, something went wrong. Please try again and make sure ...
MEM Non-Essential Amino Acids Solution, 100X Gibco 11140050 Millex-HV Syringe Filter Unit, 0.45 µm, PVDF, 33 mm, gamma sterilized Millipore SLHV033RB Nalgene General Long-Term Storage Cryogenic Tubes Thermo Scientific 5000-1020 Nunc Cell Culture/Petri Dishes Thermo Scientific 171099 O...
Because expression of the HCV viral polyprotein is an essential early step in the HCV life cycle and current systems for the study of HCV protein and host cells interactions during early phase of viral infection are limited, the regulated HCV ORF expression in HH4 cells provides a reasonable ...
The gRNA has been found to play essential roles in CRISPR systems, thus representing an attractive target to chemical manipulation17,27,28,29. In a previous study, Bhatia and co-workers have developed a robust light-activated CRISPR system30. However, to perform such regulation, additional protec...
Two constructs (N270/F271 and N273/R274) split BirA* in a way that separates the C-terminal domain that was previously described as essential for the activity of BirA15. Another construct segregates the N-terminal DNA-binding domain (Q65/L66), which was also reported to stimulate activity...
Taken together, these results indicate that a region of HIF-1 alpha spanning residues 526-641 was essential for physical interaction with VHL. Interestingly, this region contains the oxygen/redox-dependent degradation domain of HIF-1 alpha which has previously been demonstrated to mediate proteasomal ...