◆Types ofTurboKnockout®Mouse Services Conventional knockout In conventional knockout models, one or more critical exons of the target gene are replaced with a drug-selection cassette. This results in the permanent inactivation of the target gene in all cells of the body throughout development....
In conditional knockout mice, a gene is inactivated in a specific tissue to study the function of individual genes and model human diseases. Contact us for more conditional knockout strategy.
We have previously shown that N-WASP is critical for cell-ECM adhesion in fibroblasts. To characterize the role of N-WASP in fibroblast for skin development, we generated a conditional knockout mouse model in which fibroblast N-WASP was ablated using the Cre recombinase driven by Fibroblast ...
JAK2 V617F impairs hematopoietic stem cell function in a conditional knock-in mouse model of JAK2 V617F鈥損ositive essential thrombocythemia & Green, A.R. ( 2010 ) JAK2 V617F impairs hematopoietic stem cell function in a conditional knock-in mouse model of JAK2 V617F-positive essential ...
Cell culture experiments and immunofluorescence further showed that the knock-in FGFR2 M391R allele functions like that of BBDS, aberrantly translocating FGFR2 to the nucleus. Moreover, generation of a Wnt1-Cre; FGFR2 M391R /+ mouse renders a phenotype, craniosynostosis, along with other ...
15 - 18 Given the high frequency of PIK3CA mutations and ongoing efforts to develop small-molecule inhibitors, a knock-in mouse model that can express endogenous levels of mutant Pik3ca from its native promoter and genomic architecture would be a valuable tool to study tumor initiation, ...
In the conditional knockout mouse model, the gene of interest is deleted only in selected cells, as determined by a tissue-specific promoter. Conditional knockout mice are usually generated using Cre-LoxP, Dre-Rox, or Flp-Frt recombination systems for permanent gene alterations in specific tissues...
The conditional knockout (cKO) mouse model is a powerful tool for investigating disease mechanisms and is specifically used to study specific gene functions among related human disease pathologies. While traditional knockout (KO) mice have a gene disabled throughout the organism, conditional knockout...
CRISPR/Cas9-based genome editing can easily generate knockout mouse models by disrupting the gene sequence, but its efficiency for creating models that require either insertion of exogenous DNA (knock-in) or replacement of genomic segments is very poor. The majority of mouse models used in researc...
The majority of mouse models used in research involve knock-in (reporters or recombinases) or gene replacement (e.g., conditional knockout alleles containing exons flanked by LoxP sites). A few methods for creating such models have been reported that use double-stranded DNA as donors, but ...