The two groups of non-dechorionated eggs were put in a nylon mesh bag and given a distilled water wash. The chosen gut isolates were cultured overnight in Luria-Bertani (LB) broth medium at 30 °C with shaking, and castor leaves were soaked in different bacterial solutions with the same...
altered the composition and ratio of sterols in its cell membrane, and affected its multiple tolerance. The results suggest that the knockout of theErg5can enhance the thermotolerance ofK. marxianus
Sweetpotato, Ipomoea batatas (L.) Lam., is an important food crop widely grown in the world. However, little is known about the genome of this species because it is a highly heterozygous hexaploid. Gaining a more in-depth knowledge of sweetpotato genome
Aliquots of one ml of the grown culture was subsequently transferred into a number of 250 ml Erlenmeyer flask containing 50 ml of LB broth and serially incubated at temperatures 50, 55, 60, 65 and 70 °C for isolation of thermophilic bacteria. A suitable volume (100–400 μl) of each ...
The supernatants obtained were water bathed at 80 °C for 20 min and then inocu- lated to the broth medium. Then, the mixture was cul- tured at 200 rpm and 37 °C for 24 h. Culture broth of appropriate concentration was spread on the solid acid- producing bacteria ...
Results The growth resumption delay of a ΔrelA strain is dependent on the outgrowth medium and can be abolished by the addition of the complete set of 20 amino acids. We used two standard types of microbiology media: chemically defined minimal medium M925 and complex Lysogeny Broth (LB)...
in luria broth (LB) plus 0.1% glucose. Cell pellets were resuspended in buffer A [20 mM sodium phosphate (pH 7.4), 0.5M NaCl, 20 mM imidazole] and sonicated to prepare a cell lysate. The clarified lysate was loaded onto a 1 mL HisTrap™ column. The column was washed with 90 ...
No. 13/948,446 is directed to a method of modulating LDL levels, HDL levels, cholesterol levels, and triglyceride levels in a subject comprising administering to the subject in need of such treatment a pharmaceutical composition comprising a therapeutically effective amount of active α1PI, thereby...
A number of authors have linked the application of LAB probiotics with beneficial effects in models of gastrointestinal infection using small animals. Ogawa et al. (2001) reported that the use of Lb. casei Shirota reduced colonization levels and decreased the severity of diarrhoea in E. coli ...
The present invention also provides an expression vector for preparing the aforesaid active ingredient; wherein said expression vector comprises a plasmid; wherein said plasmid comprises: a nucleotide sequence comprising at least one sequence selected from a group consisting of SEQ ID NO: 05, SEQ ID...