The separation of mast cells, large cells with a high density, was also performed using an eluent composed of RPMI 1640 medium+10% FCS. Mast cells were satisfactorily separated from other cell components under a high speed revolution combined with a low speed of coil rotation (2–5 rpm)....
The human leukemia cell line HB1119 (ref. 3) was cultured in RPMI 1640 medium, supplemented with 10% fetal bovine serum (FBS) and penicillin–streptomycin (PS). The 293T and 293TN (System Biosciences) cell lines and iMEFs57 were cultured in DMEM medium supplemented with 10% FBS and PS...
The immortalized murine RAW264.7 macrophages and BV2 microglial cells were maintained at 5 × 105 cells/mL in 100 mm dishes in RPMI1640 medium supplemented with 10% (v/v) heat-inactivated FBS, penicillin G (100 units/mL), streptomycin (100 mg/mL), and L-glutamine (2 mM), and cul...
Briefly, the total splenocytes, isolated NK cells, or NK cell-depleted splenocytes were pre- pared in RPMI-1640 medium as effector cells. Aliquots (100 µL) of each cell suspension were plated onto round-bottom 96-well plates (3 wells per group), with 50 µL WEHE at vari- ...
Contaminating erythrocytes were lysed and cells (1 × 106/ml/well) were cultured in 24 well plates using complete RPMI 1640 basal medium, 10% fetal calf sera, supplemented with 20 ng/ml GM-CSF with regular media changes every 3 days. Flow cytometry was used to confirm the expression of ...
(B) Quantitative analysis of ingredients in CS aqueous extract (both pretreatment and posttreatment). Abbreviations: Thea, theanine; CAF, caffeine; C, catechin. 2.2. Cells and reagents The 4T1 cells were cultured in RPMI-1640 medium containing 10% (vol/vol) fetal bovine serum and 1% ...
(10 g/L yeast extract, 20 g/L bactopeptone, 20 g/L glucose) was used. For all other experiments, the cell suspension measured at OD600, was made in Roswell Park Memorial Institute-1640 (RPMI) medium (Sigma-Aldrich), and prepared in accordance with CLSI guidelines25. Briefly, the...
Human T-lymphoid cells Jurkat that are susceptible to FasL-mediated apoptosis were suspended in RPMI 1640 medium (2 × 106cells/mL). Aliquots of Jurkat cells (50 μL) were incubated for 24 hours at 37°C with (1) blood components' supernatant (50 μL); (2) anti-Fas apoptosis inducing...
For the MTT assay, one hundred μL sample of five different concentrations (6.25 - 100 μg/mL) of the samples were filled into a 96 well round bottom microplate containing 100 μL cells suspension in RPMI 1640 10% FCS. The plates were incubated for 24 h at 37°C and 5% CO2. Twenty...
Subclones of the T cell hybridoma cell lines, D0-11.10 (CD4+, H2d restricted, OVA-specific) and RF3370 (CD8+, H2Kb restricted, OVA specific) were maintained in RPMI 1640 (Life Technologies, Grand Island, NY) supplemented with 10% heat inactivated bovine calf serum, 2 mM glutamine, 1 ...