The separation of mast cells, large cells with a high density, was also performed using an eluent composed of RPMI 1640 medium+10% FCS. Mast cells were satisfactorily separated from other cell components under a high speed revolution combined with a low speed of coil rotation (2–5 rpm)....
The immortalized murine RAW264.7 macrophages and BV2 microglial cells were maintained at 5 × 105 cells/mL in 100 mm dishes in RPMI1640 medium supplemented with 10% (v/v) heat-inactivated FBS, penicillin G (100 units/mL), streptomycin (100 mg/mL), and L-glutamine (2 mM), and cul...
3) was cultured in RPMI 1640 medium, supplemented with 10% fetal bovine serum (FBS) and penicillin–streptomycin (PS). The 293T and 293TN (System Biosciences) cell lines and iMEFs57 were cultured in DMEM medium supplemented with 10% FBS and PS. To exclude the effects of the mosaic ...
(Scotland); endothelial growth factor from Nerliens Meszansky (Oslo, Norway); penicillin and streptomycin and EC growth medium (EGM-2MV) from Lonza (Walkersville, MD, USA); and MCDB 131, RPMI-1640 and DMEM medium with Glutamax was provided by Life TechnologiesTechnologies (NY, USA); fetal...
Monocytes were cultured in RPMI 1640 medium (Life Technologies) supplemented with fetal bovine serum (FBS, Greiner Bio-one), 1 mM Ultra-glutamine (BioWhittaker), antibiotics (100 U ml−1 penicillin, 100 μg ml−1 streptomycin and 0.25 μg ml−1 amphotericin B, Gibco)...
NB cell lines previously shown to be Mcl1 dependent (IMR5,58NLF), Bcl2 dependent (SMS-SAN, NB-164359) and resistant (SK-N-AS, BE2C) were used. Cells were grown in RPMI-1640 (Life Technologies, Grand Island, NY, USA) supplemented with 10% fetal bovine serum, 2 mM L-glutamine,...
The purity of each component was ≥ 98% by HPLC analysis. Roswell Park Memorial Institute (RPMI) 1640 medium, Radio Immunoprecipitation Assay (RIPA) Lysis Buffer, Thiazolyl Blue Tetrazolium Bromide (MTT) and trypsin–EDTA were obtained from Beijing Solarbio Sci- ence & Technology Co., Ltd ...
Transfections of plasmids into cells were carried out by using PolyJetTM (SignaGen) according to the manufacturer’s protocol. Cell culture Dulbecco’s modified eagle medium (DMEM), roswell park memorial institute-1640 (RPMI-1640) (1×), and fetal bovine serum (FBS) were purchased from ...
Isorhamnetin and dasatinib for cell viability assays were prepared in DMSO (20 mM) and diluted to different concentrations with complete RPMI-1640. The end concentration of DMSO was < 0.1%. Qingdai (2.5 g) was extracted with 25 ml CHCl3 (with 2% chloral hydrate) for 60 min using...
A 200 to 250 g guinea pig was injected with 1 mL of thioglycolate medium, after 20 hours adherent macrophages derived from abdominal cavity (gMφ) were collected by aid of RPMI 1640, suspended in RTAG, then 0.9 mL of solution was pipetted into thirteen Falcon 12-well culture plates. The...