protozoa parasites; culture media; RPMI-PY medium; Leishmania spp.; Trypanosoma cruzi1. Introduction Hemoflagellates include parasitic protozoa of medical relevance to humans and other vertebrates. Leishmania and Trypanosoma are the two genera in the group that have a global impact on human health [...
Advanced Minimum Essential medium (A-MEM), heat-inactivated fetal bovine serum (FBS), GlutaMAX™ Supplement, TrypLE™ Select, Hank’s balanced salt solution (HBSS), and 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES) were purchased from Thermo Fisher Scientific (Waltham, MA, USA)...