The practical described here is an investigation into the two types of cell death: necrosis (induced by sodium azide) and apoptosis (induced by sodium chromate) that has been delivered to undergraduate students
Apoptosis vs. Necrosis Archaea vs. Bacteria Aristotle vs. Plato Artery vs. Vein Asexual reproduction vs. Sexual reproduction Asteroid vs. Meteor Autotrophs vs. Heterotrophs Virus vs. Bacteria Wasp vs. Bee Bison vs. Buffalo Moth vs. Butterfly : What is the difference between a moth and a but...
Human Cell (2023) 36:2027–2039 https://doi.org/10.1007/s13577-023-00963-x RESEARCH ARTICLE The effect of local anaesthetics on apoptosis and NETosis of human neutrophils in vitro: comparison between lidocaine and ropivacaine Karolina Iwona Kulińska1 · Sandra S...
The test can also stain cells that are in the late stage of necrosis, albeit less intensely than apoptotic cells.25 We also demonstrate that the phosphorylation of P70 S6 kinase and P44/42 MAPK after supercooling and rewarming was higher overall in ECs compared with SMCs, which supports our ...
To investigate the mode of cell death upon treatment with GOs, the cells were exposed to equal concentrations of GOs and stained with Annexin V-FITC and PI for flow cytometric analysis. The degree of necrosis and apoptosis increased very slowly with decreasing SLGO size (Fig. 2C and Supplement...
Apoptosis Ischemia causes the destruction of cardiomyocytes and leads to necrosis. However, the infarct size of myo- cardium is determined not only by the necrosis after ischemia, but also the apoptotic cell death triggered and principally developed during the phase of reperfusion. Apoptosis is ...
Objective To explore the relationship between DJ-1,DJ-1M26I,DJ-1L166P with the cell proliferation and apoptosis of NIH 3T3 cells at cellular level,and provide a basis for the construction of a transgenic animal model of Parkinson's disease and further study on the pathogenesis of this disease...
Octenisept® was considered suitable, due to its broad spectrum of antiseptic efficacy, but, within a few months, four patients presented severe tissue necrosis. Repeated surgery and debridement were required in all patients. Wound healing was prolonged and the patien...
Detection of apoptosis and necrosis by Annexin V/PI double staining and confocal microscopy The early and late apoptotic D24 cells were detected using the Annexin V-FITC kit (Beckman Coulter, USA), according to the manufacturer's instructions with slight modifications. After treating the D24 ...
Apoptosis and necrosis assay BxPC-3 and MIA PaCa-2 cells were seeded in 24-well plate (15,000 cells/well) and incubated at 37°C in a humidified atmosphere containing 5% CO2. After 24 h, 90%, 50%, and 10% of EC50 of NSAIDs were added to the wells and incubated for 72 h. Then...