For clones from the RNA library, 21.5% of RNA clones did not indicate close relatives in the DNA library. Based on the comparisons between DNA and RNA libraries, our data indicate that the characterization of the bacterial community based on RNA has the potential to characterize distinct ...
The RNA-based method, while was superior for detection of live bacterial cells still identified a number of 16S rRNA targets in samples spiked with dead cells. In environmental water samples, the DNA- and PMA-based approaches perhaps overestimated the richness of microbial community compared to ...
Li, R., Tun, H.M., Jahan, M.et al.Author Correction: Comparison of DNA-, PMA-, and RNA-based 16S rRNA Illumina sequencing for detection of live bacteria in water.Sci Rep
Types OF RNA 1. r-RNA 2. t-RNA 3. m-RNA View Solution Molecular Basis OF Inheritance;RNA Structural Parts OF RNA Types OF RNA 1.R-RNA 2.M-RNA 3.T-RNA View Solution RNA Structural Parts OF RNA RNA World Comparison Between R-RNA M-RNA and T-RNA Questions Based on DNA and RNA ...
From the analysis of such clusters, information on the community diversity, richness and evenness can be derived13, while accounting for the degree of divergence between different ecosystems or sample types14. However, the choice of primers used to amplify 16S rRNA leads to potential biases in ...
摘要: The analytical procedure for the quantification of DNA and RNA described is the quantitative visual comparison of an unknown sample with known standards. This is quick and easy to do, suitable for amounts of sample as small as 10 ng. Keywords: DNA; RNA; rapid quantification关键词:...
We describe the isolation of two recombinant λ phages, each containing genomic DNA fragments encoding both the major adult α- and β-globin mRNAs of X. l... RK Patient,JA Elkington,RM Kay,... - 《Cell》 被引量: 128发表: 1980年 Intron function in the nonsense-mediated decay of β-...
This difference can be accounted for by interactions between the preQ(1) methylamine and base G5 of the aptamer. To explore conformational states in the absence of metabolite, the free-state aptamer structure was determined. A14 from the ceiling of the ligand pocket shifts into the preQ(1)-...
we calculated the Ka/Ks ratios for some representative species of vertebrates (Fig.1B). The Ka/Ks ratios between the species of all of the genes were < 1, suggesting a deviation from neutral expectations (Ka/Ks = 1) and negative Darwinian selection during the evolution of vertebrat...
S1A,B). There was no significant difference in the contamination between assemblies using data from BGISEQ-500 and HiSeq 2000 (Fig. 1B). Figure 1 Quality assessment of genome assemblies. (A) Completeness and (B) contamination of genome assemblies generated from BGISEQ-500 sequencing data and ...