colony formation assay原理 Colony Formation Assay: Understanding the Fundamentals and Applications Introduction The colony formation assay, also known as a clonogenic assay or colony forming unit (CFU) assay, is a fundamental technique used in molecular biology and cancer research to evaluate cell ...
The broadly accepted colony formation assay (CFA) directly measures a cell’s ability to divide; however, it takes weeks to perform and is incompatible with high-throughput screening (HTS) technologies. Here, we describe the MicroColonyChip, which exploits microwell array technology to create an ...
Next, we determined the impact of the drug treatments on cell survival using the colony formation assay. Transfection with MGMT has a dramatic impact on MMS and MNNG sensitivity as determined by the clonogenic survival assay. While the parental and neomycin resistant CHO controls show decrease of ...
54 In addition to their stimulation of growth and migration, G-CSF and GM-CSF may also contribute to this angiogenic reaction by stimulating endothelial cell proliferation and migration, as shown in cell culture and the rabbit cornea assay.30, 31 The immunohistochemical identification of both ...
After 36 hours, cell viability was measured using the MTT assay. Treatment with G-CSF at concentrations up to 100 ng/mL had no cytotoxic effects on HepG2 cells. However, treatment with G-CSF at a concentration of 1000 ng/mL reduced cell viability, as observed by comparisons with the...
Marine nitrogen fixation is a key process that stimulates primary production in the N-depleted surface ocean, thereby influencing global carbon and nitrogen cycling [1, 2]. First observed by mariners who referred to it as “sea sawdust,” the colonial cyanobacterium Trichodesmium is now recognized ...
The gold standard assay for radiation response is the clonogenic assay, a normalized colony formation assay (CFA) that can capture a broad range of radiation-induced cell death mechanisms. Traditionally, this assay relies on two-dimensional (2D) cell cul