(57) How to recombine a library of nucleic acid that has been [summary] codon change is provided. The nucleic acid, as compared to the wild type sequence, in addition to codon changes and modifications may include non-conservative or conservative modifications of the coding sequence. Also a ...
One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces The RNA-guided DNA editing technology CRISPRs (clustered regularly interspaced short palindromic repeats)/Cas9 had been used to introduce double-stranded b... H He,G Zheng,W Jiang,... - 《生物化学与生物物理学报(英文)...
Silent Mutation: No codons are changed so as to alter the polypeptide sequence of the protein product of a gene. Missense Mutation: At least one codon is altered so as to change its coded amino acid, thus altering ...
In the present study, by using the minor form of brazzein as the basic sequence, the codon-optimized brazzein gene was designed based on appropriate GC content and preferred codon of E. coli . Then, the synthesized brazzein gene was cloned in pET28a vector and expressed in BL21(DE3) and...
Codon change statistics for GMV start site changes in medium and high diversity genome test sets.Michael, E. WallSindhu, RaghavanJudith, D. CohnJohn, Dunbar
(57) How to recombine a library of nucleic acid that has been [summary] codon change is provided. The nucleic acid, as compared to the wild type sequence, in addition to codon changes and modifications may include non-conservative or conservative modifications of the coding sequence. Also a ...
(1995) Codon- dependent conformational change of elongation factor Tu preceding GTP hydrolysis on the ribosome. EMBO J., 14, 2613±2619.Rodnina MV, Fricke R, Kuhn L, Wintermeyer W (1995) Codon-dependent conformational change of elongation factor Tu preceding GTP hydrolysis on the ribosome. ...
Effect of a change of unusual initiation codon TTG to ATG by site-directed mutagenesis on -y-glutamyl cysteine synthetase gene expression. Agric. Biol. Chem. 51:1111-1114.Watanabe, K., K. Murata, and A. Kimura. 1987. Effect of a change of unusual initiation codon TTG to ATG by site-...
A synonymous codon change in the LMNA gene alters mRNA splicing and causes limb girdle muscular dystrophy type 1B 喜欢 0 阅读量: 29 作者: Todorova,A 摘要: History seems to show us that any definition of intellectual disability (ID) including our current one has no timeless, certain ...
Codon usage bias has been described for various organisms and is thought to contribute to the regulation of numerous biological processes including viral infections. HIV-1 codon usage has been previously shown to be different from that of other viruses and man. It is evident that the ...