CODON-OPTIMIZED GENES FOR THE PRODUCTION OF POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS YEASTSThe present invention relates to fatty acid desaturases and elongases able to catalyze the conversion of linoleic acid (LA) to γ-linolenic acid (GLA); α-linoleic acid (ALA) to stearidonic acid (STA);...
The clear advantage of using this protocol is that the assembled gene is without the mutation and deletion that are usually of major problem in constructing synthetic genes. The synthetic GADD34533-632 gene was cloned, expressed, and purified in large quantity. We obtained approximately 50 mg of...
Unexpected expression of intermediate filament protein genes in human oligodendroglioma cell lines Neuropathol. Exp. Neurol., 54 (1995), pp. 23-31 CrossrefView in ScopusGoogle Scholar 34. Y. Saeki, X.O. Breakefield, E.A. Chiocca Improved HSV-1 amplicon packaging system using ICP27-deleted, ...
Exogenous genes typically used as a transformation marker cannot be effectively transcribed in the macronucleus under an exogenous promoter. In addition, control of the expression level of injected genes may be difficult in transformation by native gene injection. One approach to these problems is to...
genes of the Human origin. Codon bias is due to the differences in the codon usage in the bacterial genome and the human genome. The codons present rarely in the genome ofE. coliare bound to have lesser amount of t-RNA and the scarcity of the t-RNA can cause the premature termination...
Moreover, the generation and/or function of iPSCs derived from patients carrying an acute or latent infection with EBV (a common occurrence) may be affected by virus-induced overexpression of EBNA1, which in turn could lead to a hyper-expression of the exogenous reprogramming genes33. The use...
Article: Plant codon optimized cry genes of Bacillus thuringiensis can be expressed as soluble proteins in Escherichia coli BL21 Codon Plus strain
Codon optimization of the HIV-1 vpu and vif genes stabilizes their mRNA and allows for highly efficient Rev-independent expression Two HIV-1 accessory proteins, Vpu and Vif, are notoriously difficult to express autonomously in the absence of the viral Tat and Rev proteins. We examined ... KL...
In an attempt to boost the expression of oriHERV-K113, a reconstituted HERV-K(HML-2) element, we codon-optimized large sections of the genes coding for Gag, Pro, Pol and Env. Regions containing the presumed packaging site, slippery sites, splice sites and the Rec-responsive element were ...
In an attempt to boost the expression of oriHERV-K113, a reconstituted HERV-K(HML-2) element, we codon-optimized large sections of the genes coding for Gag, Pro, Pol and Env. Regions containing the presumed packaging site, slippery sites, splice sites and the Rec-responsive element were ...