Long non-coding RNAs (lncRNAs), a kind of non-coding single-strand RNAs, play an important role as carcinogenic genes or tumor suppressors in the development of human cancer. Myocardial infarction-associated tr
The sequence(顺序) of the coding strand (编码链:双链DNA中,不能进行转录的那一条DNA链,该链的核苷酸序列与转录生成的RNA的序列一致(在RNA中是以U取代了DNA中的T),又称有义链(sense strand)。)of DNA is the same as the A. template strand.B. mRNA. C. tRNA. D. B. oth of theseE C. tRNA...
nano-barcodingshorter RNA fragmentstopological transformationuniversal codingDisease diagnosis and prognosis through the detection of genetic biomarkers occupy an important part in molecular biology. Considering the thermodynamics of the strand銉緄splacement reaction, we studied a system that can convert ...
A. et al. A strand-specific switch in noncoding transcription switches the function of a Polycomb/Trithorax response element. Nat. Genet. 46, 973–981 (2014). Article CAS PubMed PubMed Central Google Scholar Barlow, D. P. & Bartolomei, M. S. Genomic imprinting in mammals. Cold Spring...
(lincRNA; located between protein-coding genes and at least 1 kb away from the nearest protein-coding genes), intronic (located within the intron of annotated protein-coding genes), anti-sense (RNA molecules that are transcribed from the opposite strand of many protein-coding genes), and ...
Using poly(A)-independent and strand-specific RNA-seq, we identified 1,500 to 1,800 lncRNAs expressed in each of the following tissues of Brown Norway rats: the renal cortex, renal outer medulla, liver, cardiac left ventricle, adrenal gland and hypothalamus. Expression and the binding of ...
Negative frames indicate that the predicted regions are on the reverse complement strand. 3. Length: The length of the predicted region in amino acids 4. From: The position of the first/last amino acid in the translated 5. To: nucleotide sequence of the reference sequence starting with 1. ...
First-strand cDNA reverse transcription from mRNA was performed using the iScript cDNA Synthesis Kit (BioRad, Hercules, CA, USA) in 20-μL reactions with 2 μL of total RNA, according to the manufacturer’s recommendations. The reaction was performed at 25 °C for 5 min, 42 °C for 30...
RNA therapeutics (RNATx) aim to treat diseases, including cancer, by targeting or employing RNA molecules for therapeutic purposes. Amongst the most promising targets are long non-coding RNAs (lncRNAs), which regulate oncogenic molecular networks in a cell type-restricted manner. lncRNAs are distinct...
We hypothesised that tumour SNVs could be simulated by wild-type Cas9 protein, which is known to cause similar mutations when double-strand breaks are resolved by error-prone DNA repair18,51. We selected six regions of NEAT1, based on high mutation density, evolutionary conservation and known...