Recommended Service: Immunoprecipitation (IP) Why: IP is ideal if your objective is to isolate and analyze a specific protein, enabling you to focus on its structure or function without interference from other proteins. Suggested Applications: Structural analysis, functional assays, antibody validation,...
Compared with other methods for studying protein–protein interactions (such as pulldown assays and yeast two-hybrid screening), the protein–protein interactions identified by immunoprecipitation are generally an accurate reflection of the in vivo situation....
1、定量或者定性质谱数据；2、raw文件；3、analysis文件夹，数据分析结果；4、实验报告。文献举例 图 Co-IP实验结果展示 撰写文章时，该实验可引用以下参考文献Isono E, Schwechheimer C. Co-immunoprecipitation and protein blots. Methods Mol Biol. 2010;655:377-387.Lin JS, Lai EM. Protein-Protein Interac...
免疫共沉淀(Co-Immunoprecipitation)原理及实验方法-英文 Principle: Co-Immunoprecipitation (Co-IP) was developed from the immunoprecipitation technique with which Co-IP shares the fundamental principle of the specific antigen-antiody reaction. Co-IP helps determine whether two proteins interact or not in ...
免疫共沉淀(Co-Immunoprecipitation)原理及实验方法-英文.doc,Principle: Co-Immunoprecipitation (Co-IP) was developed from the immunoprecipitation technique with which Co-IP shares the fundamental principle of the specific antigen-antiody reaction. Co-IP hel
Protein Interaction Analysis Services Co-Immunoprecipitation (Co-IP) Co-IP is a classic technology widely used for protein-protein interaction identification and validation. Based on the specific immunological interaction between the bait protein and its antibody, co-IP has become an effective and reliab...
Whereas a number of antibody validation strategies can be used to verify the specificity of an antibody, antibody validation by immunoprecipitation followed by mass spectrometry analysis (IP-MS) can also identify previously known prote...
免疫共沉淀(Co-Immunoprecipitation,Co-IP)基于抗体和抗原之间的特异性/专一性作用用于研究蛋白质相互作用的金标准(gold standard)。相较于pull down、酵母双杂交等研究蛋白互作的方法,其特色之一便是捕获的蛋白互作发生在所研究的特定组织细胞内,保留了互作蛋白及复合体(complexes)的“内源”状态,但这些要能够达到IP级...
Analysis of epigenetic phenomenon RNA Immunoprecipitation (RIP)is performed with an antibody that targets a specific RNA-binding protein. By isolating the protein, the RNA bound to it is also isolated. The RNA-protein complexes are separated by RNA extraction. The RNA can be analyzed by cDNA seq...
Co-immunoprecipitation, Co-IP in short, is a widely applied technique to identify physiologically-relevant protein-protein interactions by utilizing target protein-specific antibodies to indirectly capture proteins that are bound to this specific target protein. As an extension of IP, Co-IP can capture...