结果PCR和测序证实,构建分别携带c—myc野生型及T58A突变型基因的慢病毒载体,并包装慢病毒,病毒滴度测定结果分别为6.10×10^7、5.65×10^7TU/ml。结论成功构建含有c—myc野生型及T58A突变型基因的慢病毒载体并包装出具高效感染力的慢病毒颗粒。孟春晖李福年张佃良中华实验外科杂志孟春晖,李福年,张佃良.c-myc基因慢...
结果PCR和测序证实,构建分别携带c—myc野生型及T58A突变型基因的慢病毒载7体,并包装慢病毒,病毒滴度测定结果分别为6.10×101、5.65×10TU/ml。结论成功构建含有c·myc野生型及T58A突变型基因的慢病毒载体并包装出具高效感染力的慢病毒颗粒。【关键词】c-myc基因;慢病毒;Gateway技术Constructionandsignificanceof...
Lv-BSD+c-Myc(T58A) 滴度液 货号 VGV-4609-56A5 规格 1E8 PFU 品牌:Vigen/镇江维根 货期:2～3周 赠送等量对照病毒产品说明书 相关数据 产品订购 产品名称: * 产品备注: * 称呼: * 联系电话: * 电子信箱: * 验证码: *基因&粒 现货质粒 质粒定制 病毒包装 现货病毒产品 病毒包装 细胞株 现货...
The c-Myc Transactivation Domain Is a Direct Modulator of Apoptotic versus Proliferative Signals Furthermore, the antiapoptotic survival factor insulin-like growth factor 1 was found to suppress phosphorylation of T58, suggesting that the c-Myc ... DW Chang,GF Claassen,SR Hann,... - 《Molecular...
图2 导致人类癌症中MyC激活的机制。遗传畸变,如染色体易位和基因组扩增,导致MYC mRNA表达增加。上游调节途径的改变可导致MYC癌基因转录的增加或减少。c | MYC蛋白的翻译后修饰,例如丝氨酸62(S62)残基相对于苏氨酸58(T58)的优先磷酸化,可以阻止MYC的降解并促进MYC的稳定。
[18]. The degradation of c-Myc involves a cascade of phosphorylation events, with serine 62 (S62) phosphorylated by ERK and threonine 58 (T58) regulated by GSK3β [19,20]. Additionally, other molecules, such as ubiquitin-specific peptidases 5 and 37, also participate in the degradation ...
T58 (Fig.S4). Whereas, anlotinib did not affect the interaction between c-Myc and its partner protein Max (Fig.S5). To clarify the molecular basis for the inhibition of c-Myc induced by anlotinib, we evaluated the possibility of c-Myc protein as a cellular direct target of anlotinib. ...
Further, a non-ubiquitinated mutant of c-Myc can be degraded by the proteasome in the nucleolus27. These results suggest the existence of a local ubiquitin-independent degradation pathway for c-Myc27. Our results that AZ2 destabilizes the mutant c-Myc (T58A) and accelerates degradation of ...
implying a role for proteasomal degradation of c-Myc protein in restoring c-Myc levels under Omomyc influence. Treatment with a different c-Myc inhibitor, MYCi361, also reduced c-Myc stability [94]. This destabilization effect correlated with an increase in c-Myc T58 phosphorylation by glycogen...
To test this, we overexpressed c-MYC (WT) and c-MYC (T58A) in Huh7 cells. c-MYC (T58A) is a stabilized mutant since phosphorylation on its glycosylation site Thr-58, a priming step for its degradation, is prevented (Figure S7F) (Kamemura et al., 2002). Whereas under glucose de...