结果PCR和测序证实,构建分别携带c—myc野生型及T58A突变型基因的慢病毒载体,并包装慢病毒,病毒滴度测定结果分别为6.10×10^7、5.65×10^7TU/ml。结论成功构建含有c—myc野生型及T58A突变型基因的慢病毒载体并包装出具高效感染力的慢病毒颗粒。孟春晖李福年张佃良中华实验外科杂志孟春晖;李福年;张佃良.c-myc基因慢...
Lv-BSD+c-Myc(T58A) 滴度液 货号 VGV-4609-56A5 规格 1E8 PFU 品牌:Vigen/镇江维根 货期:2～3周 赠送等量对照病毒产品说明书 相关数据 产品订购 产品名称: * 产品备注: * 称呼: * 联系电话: * 电子信箱: * 验证码: *基因&粒 现货质粒 质粒定制 病毒包装 现货病毒产品 病毒包装 细胞株 现货...
上游调节途径的改变可导致MYC癌基因转录的增加或减少。c | MYC蛋白的翻译后修饰,例如丝氨酸62(S62)残基相对于苏氨酸58(T58)的优先磷酸化,可以阻止MYC的降解并促进MYC的稳定。 图3 MYC是癌症标志的伟大协调者。MYC调节多种癌细胞固有和宿主依赖性途径,以促进癌细胞生长和存活(绿色...
The c-Myc Transactivation Domain Is a Direct Modulator of Apoptotic versus Proliferative Signals Furthermore, the antiapoptotic survival factor insulin-like growth factor 1 was found to suppress phosphorylation of T58, suggesting that the c-Myc ... DW Chang,GF Claassen,SR Hann,... - 《Molecular...
【摘要】目的 研究突变体c-myc(T58A、S62A)、WT c-myc对平滑肌细胞迁移和侵袭的调控.方法 平滑肌细胞转染T58A、S62A、WT后采用Western blot检测法、荧光探针检测法、细胞迁移和侵袭检测法,测c-myc的表达、转录活性及平滑肌细胞迁移和侵袭的程度.结果 平滑肌细胞转染T58A、S62A、WT后c-myc蛋白表达显著增加;...
In B cells, the responsive signature genes (37/50) were induced as a cohort at 4 hr. In contrast, the signature genes appeared to be induced biphasically in T cells (26/50 at 4 hr increasing to 44/50 at 14 hr). No functional or ontological rationale for the differential early versus...
implying a role for proteasomal degradation of c-Myc protein in restoring c-Myc levels under Omomyc influence. Treatment with a different c-Myc inhibitor, MYCi361, also reduced c-Myc stability [94]. This destabilization effect correlated with an increase in c-Myc T58 phosphorylation by glycogen...
T58 (Fig.S4). Whereas, anlotinib did not affect the interaction between c-Myc and its partner protein Max (Fig.S5). To clarify the molecular basis for the inhibition of c-Myc induced by anlotinib, we evaluated the possibility of c-Myc protein as a cellular direct target of anlotinib. ...
Further, a non-ubiquitinated mutant of c-Myc can be degraded by the proteasome in the nucleolus27. These results suggest the existence of a local ubiquitin-independent degradation pathway for c-Myc27. Our results that AZ2 destabilizes the mutant c-Myc (T58A) and accelerates degradation of ...
MBI包含由氨基酸残基S62和T58组成的典型磷酸位点,通过级联磷酸化反应调节C-Myc蛋白的稳定性。MBII是C-Myc TAD中研究最广泛的部分,该部分被认为是Myc生物活性的基础,在体内,MBII对于C-Myc的致癌性十分关键。中间区域MBIII主要负责调节蛋白质的稳定性,促进C-Myc细胞转化。MBIV结构域在体外不同细胞模型的测定具有...