In addition, five surface specimens were collected: three in contact with children's saliva (spoon, child's cheek, washcloth) and two in contact with children's urine (diaper changing table, mother's hand). Samples were tested by PCR and viral culture to quantify the presence of CMV DNA ...
To detect the virus itself, in patients who are symptomatic, the sample may be blood, urine, sputum, amniotic fluid, cerebrospinal fluid, duodenal fluid, other body tissues, or saliva in newborns. Some samples, such as amniotic fluid, cerebrospinal fluid, or body tissue (biopsy), may require...
CMV nucleic acid testing (NAT), such as PCR, is one of the most common tests performed in clinical virology laboratories to identify CMV reactivation-however, there are important limitations of the test to consider. CMV-specific immune system monitoring for management of cytomegalovirus in HSCT Con...
(Applied Biosystems). Each PCR was performed in a volume of 50 μl as follows: 0.5 μg (blood) or 0.2 μg (urine) template DNA (5 μl), 5 μl 10 × DreamTaq™ Buffer (20 mM Tris-HCl, pH 8.0; 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Noni...
The BioPerfectus Human Cytomegalovirus Real Time PCR Kit is intended for use with a multiplex real-time fluorescence analyzer to detect quantitative cytomegalovirus (CMV) DNA in human urine and serum. Both EBV and HCMV can cause serious complications, especially during pregnancy or in individuals wi...
Pentamer complex PCR: Polymerase chain reaction PFU: Plaque forming unit PGM: Personal Genome Machine References Britt W. Manifestations of human cytomegalovirus infection: proposed mechanisms of acute and chronic disease. Curr Top Microbiol Immunol. 2008;325:417–70. CAS PubMed Google Scholar ...
[20]. Typically, a noncontrasted head CT is performed and abnormalities are found only a quarter of the time [21]. Infectious etiologies outside of the broad culturing (tracheal aspirate, blood, and urine culture) should always be considered in the work up of alerted mental status in an ...
Polymerase chain reaction (PCR) amplification was used to detect cytomegalovirus (CMV) in tissue culture and in urine specimens from newborns. Synthetic ol... GJ Demmler,GJ Buffone,CM Schimbor,... - 《Journal of Infectious Diseases》 被引量: 900发表: 1989年 Prenatal diagnosis of congenital cy...
Real-time PCR of newborn saliva specimens has been shown to be highly sensitive and specific compared to culture based methods for CMV screening. Although both saliva and urine samples are known to be acceptable for identifying infants with cCMV, it is thought that urine samples may contain ...
Pentamer complex PCR: Polymerase chain reaction PFU: Plaque forming unit PGM: Personal Genome Machine References Britt W. Manifestations of human cytomegalovirus infection: proposed mechanisms of acute and chronic disease. Curr Top Microbiol Immunol. 2008;325:417–70. CAS PubMed Google Scholar ...