A HUMAN CYTOMEGALOVIRUS (HCMV) INTRON A FRAGMENTThis invention relates to a Cytomegalovirus (CMV) Intron A fragments for expressing gene products are disclosed. Also described are expression vectors including the fragments as well as methods of using the same.SELBY MARK...
Effect of intron A from human cytomegalovirus (Towne) immediate-early gene on heterologous expression in mammalian cells A 2.4 kb fragment of hCMV (Towne strain), containing the 5' end of the major immediate-early gene, has been cloned, sequenced, and used to construct a seri... BS ...
英文别名: pAdenoVator-hCMV-Intron transfer vector中文名: 中文别名: CBNumber: CB8997533 分子式: 分子量: 0 MOL File: Mol file 化学性质 安全信息 用途 供应商 1 pAdenoVator-hCMV-Intron transfer vector 化学性质 安全信息pAdenoVator-hCMV-Intron transfer vector 性质、用途与生产工艺 pAdenoVator-...
水稻条纹病毒(Rice stripe virus,RSV)NS2 基因和 NS3 基因 编码的蛋白均具有沉默抑制子功能,将串联的 NS2+NS3 分别正反向插入含有 1 个 intron 的 RNAi 载体,转化水稻获得 31 株 T0 代转基因水稻植株,接种 RSV 发现,转基因水稻发病时间推迟,病毒积累量下降[7]。目前,利用 dsRNA 技术 沉默 CMV 沉默抑制子 ...
(i/r)astemplate,thenMPandRepwereconcatenatedandclonedintopGEM-TEasyVector.SenseMP—RepfragmentwasobtainedbydigestingwithBamHIandKpnIandinsertedintovectorpKAN—In,thencalledtherecombinantplasmid+pKAN.AntisenceMP-RepgenecutwithXba1waslinkedwith+pKANtoconstructhairpinstructure.ThefragmentincludingIntron,thesenseand...
If RT-PCR is used for the detection of viral transcripts differentiation of mRNA- and DNA-derived PCR products is necessary. For spliced transcripts, like the IEA mRNA, the exon-intron structure can be used. However, mRNA species encoding the late structural proteins are almost exclusively encode...
After integration into genomic DNA, this promoter transcribes a long transcript with an intron in the 5’UTR flanked with splice donor and acceptor sites derived from the lentiviral vector. Further studies found that additional CpG mutations in the MSCV LTR reduced transcriptional silencing in ...
CTCFBindingtotheFirstIntronoftheMajorImmediateEarly(MIE)GeneofHumanCytomegalovirus(HCMV)NegativelyRegulatesMIEGeneExpressionandHCMVReplicationFranciscoPuertaMartínez,aRuthCruz,aFangLu,bRobertPlasschaert,cZhongDeng,bYiselA.Rivera-Molina,aMarisaS.Bartolomei,cPaulM.Lieberman,bQiyiTangaDepartmentofMicrobiology/RCMIProg...
After integration into genomic DNA, this promoter transcribes a long transcript with an intron in the 5’UTR flanked with splice donor and acceptor sites derived from the lentiviral vector. Further studies found that additional CpG mutations in the MSCV LTR reduced transcriptional silencing in ...
CMV immediate early enhancer/promoter 1–742 Chimeric intron 857–989 T7 RNA polymerase promoter (–17 to +3) 1033–1052 NanoLuc® protein coding region 1065–1577 Linker 1578–1589 SgfI 1590–1597 Barnase coding region 1621–1956 PmeI 1958–1965 SV40 late poly(A) region 2117–2338 SV40 ea...