To meet the requirements of the biopharmaceutical industry, improving the yield of recombination therapeutic protein (RTP) from Chinese hamster ovary (CHO) cells is necessary. The human cytomegalovirus (CMV) promoter is widely used for RTP expression in
However, the MPSV promoter resulted in the highest levels of stable protein expression in CHO-K1 cells. Using the CMV driven constitutive promoters in the presence of intron A, we have been able to generate >10 渭g/ml of recombinant protein using transient transfections....
www.nature.com/scientificreports OPEN received: 30 July 2015 accepted: 22 October 2015 Published: 19 November 2015 CMV promoter mutants with a reduced propensity to productivity loss in CHO cells Benjamin Moritz1, Peter B. Becker2 & Ulrich Göpfert1 The major immediate-early promoter ...
After integration into genomic DNA, this promoter transcribes a long transcript with an intron in the 5’UTR flanked with splice donor and acceptor sites derived from the lentiviral vector. Further studies found that additional CpG mutations in the MSCV LTR reduced transcriptional silencing in ...
After integration into genomic DNA, this promoter transcribes a long transcript with an intron in the 5’UTR flanked with splice donor and acceptor sites derived from the lentiviral vector. Further studies found that additional CpG mutations in the MSCV LTR reduced transcriptional silencing in ...
White spot syndrome virus (WSSV) immediate-early promoter one (ie1) was shown to be a stronger promoter for gene expression in insect cells compared with Cytomegalovirus immediate-early (CMV) promoter in luciferase assays. In an attempt to improve expression efficiency, a recombinant baculovirus was...
Therefore, the avidity of pMHC-TCR binding as well as promoter dominance in generating MHC-binding epitopes are the two likely selection criteria that define the winners of memory inflation, both on the side of viral antigens and on the side of host T cell clones. Time of infection and its...
The present inventors concerns vectors carrying a truncated chimeric CMV-chicken β-actin (smCBA) promoter in which the hybrid chicken β-actin/rabbit β-globin intron is greatly shortened, and their use to deliver to an operatively linked polynucleotide to host cells in vitro or in vivo, result...
CMVpromoterinplant YUXiumin,YUEWenran,WANGRuigang (CollegeofLifeSciences,InnerMongoliaAgriculturalUniversity,Hohhot010018,China) Abstract:BasedontheCMVpromotergenesequence,apairofprimerswasdesigned,andthetarget genewasamplifiedbyPCR.TherecombinantexpressionplasmidLpPCGwhichcontainedKanamycinre— sistanceandaGUSintron...
pCDH-CMV-MCS-EF1-Neo载体基本信息：载体名称: pCDH-CMV-MCS-EF1-Neo 质粒类型: 慢病毒表达载体；cDNA表达载体；双启动子载体克隆方法: 多克隆位点，限制性内切酶 启动子: CMV 载体大小: -- 5' 测序引物及序列: CMV-F :CGCAAATGGGCGGTAGGCGTG 3' 测序引物及序列: -- 载体标签: 无 载体抗性: 氨苄青霉素...