The present study investigated if the quantitative interpretation of anti-CMV IgG before allo-HSCT might serve as a novel parameter for the identification of patients at risk for CMV reactivation and worse outcome post-TX. For this purpose, a cohort of 440 allo-HSCT recipients over a period of...
PCR was performed to determine CMV viral load in the patients with positive CMV IgM; however, no CMV DNA was detected in any of these patients. Similar to the findings from cohort 1, we did not observe any associa- tion between ICI-hepatitis and CMV IgG or IgM at baseline or during ...
CMV, IgM CMV, IgG Possible Interpretation Negative Negative No current or prior infection; no immunity (person is susceptible to primary infection) Symptoms due to another cause OR immune system cannot produce adequate amount of antibody (immunocompromised) Positive Negative Recent active primary infectio...
A positive IgM Avoid foam formation in all reagents and sample types (specimens, result in combination with a low avidity index for IgG is a strong indication of calibrators and controls). a primary CMV infection within the last 4 months. Seroconversion to CMV IgM and IgG also establishes the...
The recipients were predominantly anti-CMV IgG positive, 82/86 (95.3%). We found a total of 494/1359 (36.4%) of the collected plasma samples to be CMV positive in qRT PCR. The viral load ranged from 20 copies/mL to 88 000 copies/mL, with an average viral load of ∼3 000 copies...
Virus inactivation Infectivity (IEAϩ/ mL) DNA load (copies/mL) AP (U/L) % activity Lipase (U/L) % activity AD 169† D, 139 Control 1260 92,500 23 100 59 100 CMV-IgG negative 2 s 72°C 0 105,000 1 4.4 4 6.8 2 s 65°C 0 112,000 3 13.0 7 11.9 2 s 63°C 0 86...
Preventive effect of IgG from EBV-seropositive donors on the development of human lympho-proliferative disease in SCID mice. Int J Cancer, 71 (1997), pp. 624-629 View in ScopusGoogle Scholar 16 Nadal D, Guzman J, Frohlich S, Braun DG Human immunoglobulin preparations suppress the occurrence ...
Total cell lysates from U373 transgenic cell lines or infected MRC5 cells were subjected to SDS–PAGE followed by immuno- blot analysis using the anti-gH mAb AP86 (1:5 hybridoma supernatant), polyclonal anti-gL IgG (1:1,000), and an anti-GAPDH (1:10,000; Chemicon, Billeriza, MA)....
The data analyzed, included maternal date of birth, age, gravidity- and parity-status, CMV serostatus (IgG positive/ IgM negative—seropositive patients, IgG negative/ IgM negative—seronegative patients and IgG positive/ IgM positive and low IgG avidity—patients with a primary infection), due ...
1.4 Nachweismethoden und Aussagekraft Zur Diagnostik einer CMV-Infektion stehen der direkte Virusnachweis durch Zellkultur, Antigennachweise des Virus, der Nachweis von CMV-DNA, der Nachweis von IgM- und IgG-Antikörpern bzw. der Nachweis von T-Zell-Antworten gegen CMV zur Verfügung...