IgG positive recipients from CMV seronegative donors have the worst outcomes. On the other hand, CMV IgG negative recipients from CMV seronegative donors rarely develop severe CMV-related complications. Based on this, patients with hematologic diseases who are going to receive allo-HSCT need to ...
(C) An ELISA was developed to assess human IgG concentration using capturing ELISA format, using goat-anti-human IgG capturing antibodies and goat-anti-human IgG Fc conjugated with HRP as detection antibody (Southern Biotech). (D) Fraction of wells positive for IgG after human memory B-cell ...
The fluorescence of rhodamine anti-mouse IgG and DAPI was visualized and merged using a Nikon Eclipse E-800 fluorescence microscope. B) Emetine has an early activity against HCMV. Cells were infected with pp28-luciferase HCMV Towne, and compounds were added at 0, 6, 12, 24, 36, and 48...
For detection of primary antibody binding, horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG antibody and the West Pico Chemoluminescent Substrate (Thermo Scientific) were used according to the manufacturer's instructions. Removal of N-linked oligosaccharides was carried out using recombinant...
IgG positive recipients from CMV seronegative donors have the worst outcomes. On the other hand, CMV IgG negative recipients from CMV seronegative donors rarely develop severe CMV-related complications. Based on this, patients with hematologic diseases who are going to receive allo-HSCT need to ...
For detection a 1 h incubation with the appropriate secondary antibody (anti-mouse-IgG-HRP, 1:1000 dilution in 3% BSA, 1×PBS, 0.05% Tween20, pH 7) was conducted. The binding of the specific antibody to the pentameric complex was detected using 100 μl/well TMP substrate reagent (BD ...