Djordojevic B, Lange CS: Clonogenicity of mammalian cells in hybrid spheroids: a new assay method. Radiat Environ Biophys 1990;29:31–46.Djordjevic et al., Clonogenicity of Mammaliam Cells in Hybrid Spheroids: a New Assay Method , (1989), pp. 1 16....
Ten thousand hepatocytes from each trypsinized culture were used for magnetic levitation assay in 6-well ultra-low attachment plates. On day 5, live cell imaging was carried out to record spheroids formation (red arrows, magnification × 10, upper panel). The levitated culture of hepatocytes ...
Grichnik JM, Burch JA, Schulteis RD et al (2006) Melanoma, a tumor based on a mutant stem cell? J Invest Dermatol 126:142–153 Article PubMed CAS Google Scholar Clarke MF (2005) A self-renewal assay for cancer stem cells. Cancer Chemother Pharmacol 56(Suppl 7):64–68 Article PubMe...
Samples for the colony formation assay (CFA) were prepared by seeding 105 HeLa cells/well in 24-well plates. IONP treatment was done as described above. After the incubation time, the cells were carefully washed 3 times with PBS. Cells were detached, and different dilutions of each sample ...