Embedding Spheroids in Cultrex BME for Cell Invasion and Migration Assays. MDA-MB-231 (3,000 cells/well) formed spheroids in low adhesion plates for 72 hours using reagents in the Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay. Briefly MDA-MB-231 cells ...
Drug resistance assays showed that spheroid-seeded scaffolds have much higher drug resistance than monolayer cultures, spheroids on flat substrates, or scaffolds seeded with dispersed cells. Furthermore, spheroid-seeded scaffolds demonstrated higher lactate production leading to acidosis, and higher ...
The Use of 3-D Cultures for High-Throughput Screening: The Multicellular Spheroid Model 来自 NCBI 喜欢 0 阅读量: 108 作者: Kunz-Schughart,A L.摘要: Over the past few years, establishment and adaptation of cell-based assays for drug development and testing has become an important topic in ...
1A)30. The squared aspect ratio of contact length between the two aggregates over maximum diameter was calculated to assess the spheroid fusion process. The iNPC-MSC spheroids and iEC spheroids, at different iNPC: iEC: MSC ratios of Bi-(4:2), Tri-(3:2:1), Tri-(2:2:2), and Tri...
resulting in more functional neural connections and circuitry. Like the developing brain, the neurons form layers and talk with each other via neural networks. The spheroid technology is more consistent than earlier organoids in generating the same kinds of cortex-like structures in repeated experiment...
A) Quantitation of round (spheroid) and flat (disc) colonies of HCT-116 cells grown in 3-D matrigel for 6 days with the indicated combinations of growth factors; R-Spondin1 (R), Noggin (N), EGF (E), and Wnt3a (W). Results shown are mean percent of round or flat colonies +/...
M6A levels were quantified using a colorimetric method and m6A dot blots, and functional analysis was performed using spheroid adhesion assays. We first found that increased E-cadherin expression was accompanied by decreased vimentin and Slug expression in the eutopic secretory endometrium of individuals...
(E and F) Transwell assays (E) and colony and spheroid formation showed the migration and invasion abilities (E) and the tumorigenicity in vitro and self-renewal abilities (F) of SMYD3-depleted sorafenib-resistant cells. Data are represented as mean ± SD. To test the effect of SMYD3 de...
Depletion of SMYD3 by transient siRNA, stable shRNA knockdown and the SMYD3 inhibitor BCI1 all decreased spheroid invasion and adhesion. Gene expression arrays revealed downregulation of integrin family members. Inhibition assays confirmed that invasion and adhesion of spheroids are mediated by ITGB6...
The Redox Potential Dependence of Coupled Electron Flow in Chromatophores from Rhodopseudomonas Spheroides In the present communication we have used the kinetic behaviour of the shift as an index of the high-energy state of chromatophores from Rhodopseudomonas spheroides .doi:10.1007/978-94-010-2938-...