[8]Rawstron AC, Fazi C, Agathangelidis A, et al. A complementary role of multiparameter flow cytometry and high-throughput sequencing for minimal residual disease detection in chronic lymphocytic leukemia: an European Research Initiative on CLL study. Leukemia. 2016 Apr;30(4):929-36. doi: 10....
C Representative flow cytometry scatter plots of T cells after culture with the indicated condition. P values: * <0.05; ** <0.01; *** <0.001. Full size image This fact allowed us to carry out co-cultures of TN cells with MDSCs in the presence of ibrutinib under conditions that would ...
Secondary end points at 3 months post-treatment included: MRD negativity assessed in the bone marrow by highly sensitive multiparameter flow cytometry with a level of detection o 1 CLL cell in 10 000 leukocytes;13 ORR defined as at least partial remission (PR); and safety and toxicity as ...
Consistent with the flow cytometry results, a high sensitivity to venetoclax was detected in unstimulated cells as indicated by loss of TMRE+ cells, increased Annexin V+ cells, and increased condensed nuclei (Figure 1D). The patient-specific effect of venetoclax in 2S-stimulated cells was also ...
MRD status was evaluated by central laboratory per European Research Initiative on CLL recommendations25 using 8-color flow cytometry in PB samples obtained at baseline, on day 1 of cycles 7, 10, and 13, at the end of treatment (EOT) (30 days after discontinuation), on day 1 of cycles ...
Correlation of flow cytometrically determined expression of ZAP-70 using the SBZAP antibody with IgVH mutation status and cytogenetics in 1,229 patients with chronic lymphocytic leukemia. Cytometry B Clin Cytom 2009; 76: 385–393. Article PubMed Google Scholar Kern W, Bacher U, Haferlach C,...
Each pool was then sodium bisulfite converted (Fast Epitect Bisulfite, Qiagen) following manufacture recommendations. To ensure full bisulfite conversion, two cycles of conversion were performed. The double-stranded DNA was first denatured 10 min at 98 °C and then incubated for 20 min at ...
Advances in first-line treatment of chronic lymphocytic leukemia: current recommendations on management and first-line treatment by the German CLL Study Group (GCLLSG). Eur J Haematol. 2016;96:9–18. Article PubMed Google Scholar Zelenetz AD, Gordon LI, Wierda WG, Abramson JS, Advani RH, ...
Following 30 minutes of incubation at room temperature in the dark the red cells were lysed using TQ-prep instrument and ImmunoPrep reagent (both from Beckman Coulter) according to manufacturer's recommendations. Samples were analyzed on FC500 flow cytometer (Beckman-Coulter) equipped with CXP ...
(both from Beckman Coulter) according to manufacturer's recommendations. Samples were analyzed on FC500 flow cytometer (Beckman-Coulter) equipped with CXP software version 2.1 (Beckman Coulter). Multiparametric analysis was performed with gating strategy based on CD45 staining and light side scatter ...