[8]Rawstron AC, Fazi C, Agathangelidis A, et al. A complementary role of multiparameter flow cytometry and high-throughput sequencing for minimal residual disease detection in chronic lymphocytic leukemia: an European Research Initiative on CLL study. Leukemia. 2016 Apr;30(4):929-36. doi: 10....
[13] Soliman DS, Al-Kuwari E, Siveen KS, et al. Downregulation of Lymphoid enhancer-binding factor 1 (LEF-1) expression (by immunohistochemistry and/ flow cytometry) in chronic Lymphocytic Leukemia with atypical immunophenotypic and cytologic ...
C Representative flow cytometry scatter plots of T cells after culture with the indicated condition. P values: * <0.05; ** <0.01; *** <0.001. Full size image This fact allowed us to carry out co-cultures of TN cells with MDSCs in the presence of ibrutinib under conditions that would ...
Secondary end points at 3 months post-treatment included: MRD negativity assessed in the bone marrow by highly sensitive multiparameter flow cytometry with a level of detection o 1 CLL cell in 10 000 leukocytes;13 ORR defined as at least partial remission (PR); and safety and toxicity as ...
After electroporation, the induction of downstream effector caspase activity was measured by flow cytometry analysis with the use of the cell-permeable, caspase-3–specific, fluorogenic substrate CaspaTag, and the viability of the cells was determined by 7-AAD exclusion. Because each patient specimen...
The MRD assessment was performed in a single laboratory (HMDS, Leeds, UK) using multicolor flow cytometry capable of detecting minimal residual disease (MRD) to a level of one CLL cell in 10000 leukocytes as recently recommended in the IWCLL Guidelines. Patients were followed for a median ...
29 patients (47.5%) had no detectable (<10−4) minimal residual disease assessed by flow cytometry in peripheral blood. In conclusion, the BIG regimen is a safe and highly effective therapy for CLL. This is a preview of subscription content, access via your institution Access options ...
Single cells were sorted by flow cytometry into 2.5 μL of RLT Plus buffer (Qiagen) supplemented with 1 U/μL of RNase Inhibitor (Lucigen). Sorted cells were immediately stored at −80 °C. Genomic DNA (gDNA) and mRNA have been separated manually. A modified oligo-dT primer (5...
Flow cytometry was performed at baseline and designated time points. After viability assessment, samples were stained using PrepPlus2 automated staining system (Beckman Coulter) using five color whole blood staining technique with panels of directly conjugated monoclonal antibodies. Following 30 minutes of...
All patients enrolled to this study had stimulated cytogenetics, FISH, and IGHV mutational status performed at baseline as previously described (Byrd et al, J Clin Oncol (2006) 24, 437-43; Woyach et al, Br J Haematol (2010) 148, 754-9. Flow cytometry was performed at baseline and desig...