cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position ...
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for direct immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the u...
1.2.7 Western blot 法测定cleaved Caspase-3蛋白表达心肌组织经研磨、裂解后用酶标仪测蛋白浓度,根据所测得的蛋白浓度上样总蛋白质量为120 μg,经SDS-PAGE凝胶电泳分离后,采用电印迹转移法将蛋自质转至PVDF膜,丽春红S染色,根据所需目...
Cleaved Caspase-3
TheCleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Pacific Blue ™ Conjugate) AntibodyfromCell Signaling Technologyis a Rabbit Monoclonal antibody to CASP3. This antibody recognizes Homo Sapiens, Human, and Mouse antigen. TheCleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Pacific...
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RIP also interacts with TNF-receptor-associated factors (TRAFs) and can recruit IKKs to the TNF-R1 signaling complex via interaction with NEMO, leading to IκB phosphorylation and degradation (6,7). Overexpression of RIP induces both NF-κB activation and apoptosis (2,3). Caspase-8-...
This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA-binding domain (24 kDa) from the ...
Western blot analysis of extracts from COS-7 cells, untransfected (-) or transfected with construct overexpressing human caspase-1 (+), using Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb (upper) or Caspase-1 (D7F10) Rabbit mAb #3866 (lower). Western blot analysis of extracts from COS...