cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 posit...
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cleaved Caspase-3 (Asp175...
1.2.7 Western blot 法测定cleaved Caspase-3蛋白表达心肌组织经研磨、裂解后用酶标仪测蛋白浓度,根据所测得的蛋白浓度上样总蛋白质量为120 μg,经SDS-PAGE凝胶电泳分离后,采用电印迹转移法将蛋自质转至PVDF膜,丽春红S染色,根据所需目...
Cleaved Caspase-3
上海玉博生物科技有限公司 主要致力于“Cleaved Caspase-3 (Asp175) Antibody CST 9661L”的生产销售。多年的“Cleaved Caspase-3 (Asp175) Antibody CST 9661L”生产与销售的经验,与各行业新老用户建立了稳定的合作关系,我公司经营的产品名称深受广大用户信赖。欢迎来电
Nelly Béchir, Xiaomeng Zhang, Danh Tran-Thanh, Saima HassanH3- AlexaFluor 488 (Ser-10) (1:100; #3465,Cell Signaling), cleaved-caspase 3-Pacific Blue™ (1:100; #8788,Cell Signaling), and 20 µg/mL propidium iodide (PI) (#P3566,Invitrogen) with 200 µg/mLRNas...
我现在在做cleaved-caspase-3的western-blot检测,用的是10%的胶,一抗用的是Cell Signaling technology公司的,浓度为1:500,二抗用的是中衫的,浓度为1:2000,可是我做了四次了都是白板,请大家帮帮忙啊! 查看完整版本请点击这里:【求助】cleaved-caspase-3的western-blot检测我...
Immunocytochemistry analysis of Caspase 3 (Cleaved Asp175) in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114687) at a dilution of 1:200 (1 hr at 37°C...
cleaved-Caspase 3 Print datasheet MSDS Validation Data Gallery Filter: AllWBIPIHCIF WB analysis of HepG2 using 25546-1-AP Tunicamycin treated HepG2 cells were subjected to SDS PAGE followed by western blot with 25546-1-AP (Caspase 3 Antibody) at dilution of 1:600 incubated at room temperat...
This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA-binding domain (24 kDa) from th...