acnes genome, it is the housekeeping sortase of this organism. Sortase F from P. acnes shows a behavior similar to sortases from class A in terms of pH dependence, recognition sequence and catalytic activity; furthermore, its activity is independent of bivalent ions, which contrasts to sortase...
1b). This method, which we call TCR-MAP, relies on a TCR-stimulated circuit in immortalized T cells to activate sortase-mediated biotinylation of cognate APCs. Fig. 1: TCR-MAP efficiently and selectively identifies cognate human TCR–pMHC interactions. a, Schematic of the TCR-MAP antigen ...
The association of autoimmune diseases with particular allellic products of the class-II major histocompatibility complex (MHCII) region implicates the presentation of the offending self-antigens to T cells. Because antigen-presenting cells are tolerogen
Class A sortases are responsible for attachment of diverse proteins to the cell wall to facilitate environmental adaption and interaction. These enzymes are also used in protein engineering applications for sortase-mediated ligations (SML) or sortagging of protein targets. We previously investigated ...
molecules Article Discovery of a New Class of Sortase A Transpeptidase Inhibitors to Tackle Gram-Positive Pathogens: 2-(2-Phenylhydrazinylidene)alkanoic Acids and Related Derivatives Benedetta Maggio 1,*, Demetrio Raffa 1,*, Maria Valeria Raimondi 1, Stella Cascioferro 1,2, Fabiana Plescia 1, ...