Cases were confirmed by Xpert Mpox PCR and followed through hospitalisation and on days 29 and 59 after diagnosis. Of the 510 suspected cases included, 407 (80%) tested positive via PCR. Among the 407 confirmed cases, 196 (48%) were women. Age distribution was bimodal, with 58 (14%) ...
Accumulibacter” contain either the nitrate respiration gene (nar; clade IIC) or the periplasmatic nitrate dissimilation gene (nap; clades IC, IA, IIA, IIF). Only the nap gene has been previously identified in the metagenomes of enriched EBPR cultures composed of the clades IA and IC (Flower...
Once dried, 25 μL of 1 x TE was added to each tube and the extracted DNA was stored at − 20 °C until amplicon sequencing could take place. 16S rRNA gene amplicon sequencing The V3-V4 region of 16S rRNA genes were amplified with forward primer 5′ ACTCCTACGGGAGGCAGCAG 3′...