Cis -acting polymorphisms can affect expression in a tissue-specific and developmentally specific manner. They are strong candidates for positive selection and evolutionary adaptation but such variants might be
Long non-coding RNAs (lncRNAs) are diverse transcription products emanating from thousands of loci in mammalian genomes. Cis-acting lncRNAs, which constitute a substantial fraction of lncRNAs with an attributed function, regulate gene expression in a manner dependent on the location of their own sites...
Overall, the observation of multiple overlaps between the cis-acting elements of specific transcriptional pathways and GWAS-defined susceptibility loci for specific cancers, suggests that individual transcriptional pathways may often be under selective pressure in a manner that promotes the development of ...
In one embodiment, the recombinant recipient cell expresses the target nucleic acid in a manner that facilitates selection of cells comprising mutants of said target nucleic acids having a desired activity. The selection may be a direct selection for the activity encoded or otherwise determined by th...
Structure and FunctionReferences LAR Confers Position-Independent, High-Level Expression of the Human Beta-Globin Gene in Transgenic Mice LAR Confers Powerful Transcriptional Enhancing Activity LAR Activates Homologous and Heterologous Promoters in Tissue-Specific Manner LAR Is Highly Conserved in Its Organiza...
Interestingly, in vivo studies indicated that the PV oriI inhibited PV replication in a trans-dominant manner [52]. Recently a second type of internal RNA element was discovered in the coding sequence of protein 3Cpro of PV and of several coxsackie A viruses (CAVs) (Fig. 1) [53]. This ...
In contrast, the Igκ 3′ enhancer exhibits occupancy of critical factor binding sites in a developmentally appropriate manner that correlates with activation of the locus (Shaffer et al., 1997). Mice harboring deletions of either Igκ enhancer showed reduced but detectable V-to-Jκ rearrangements...
Cis-acting small peptides causing ribosome stalling in response to specific small molecules - erythromycin, chloramphenicol, tryptophan, arginine and polyamines - have been described previously [2–6,24–26]. In all these systems the ribosome is stalled on the mRNA in a manner depending on the ...
falciparum initiates translation in a cap-dependent manner similarly to other eukaryotes [39, 40]. While the central initiation factors required for cap-binding have been bioinformatically identified and many of the essential interactions have been validated, questions remain around how these factors ...
Ectopic, or mis-expression of mab-9 is deleterious, hence this gene must be tightly regulated, in both a temporal and a spatial manner. In order to identify upstream regulators of mab-9 expression, we have adopted two strategies that we hope will reveal both cis- and trans-acting factors....