A circular buffer can be written so that when the end of the allocated input buffer is reached, the pointer automatically wraps around to the beginning of the buffer. Writing to the correct memory is then ensured. This saves the time of having to check for the end of the buffer and ...
In accordance with the external business situation and future regulatory trends, as well as CCB's strategic planning, risk appetite and capital adequacy assessment, the principle for capital adequacy ratio target setting is to maintain an appropriate capital buffer based on the minimum regulatory ...
Background Extrachromosomal circular DNAs (eccDNAs) exist in human blood and somatic cells, and are essential for oncogene plasticity and drug resistance. However, the presence and impact of eccDNAs in type 2 diabetes mellitus (T2DM) remains inadequately understood. Methods We purified and sequenced...
An operation on a socket could not be performed because the system lacked sufficient buffer space or because a queue was full. An Unable to write data to the transport connectionestablished connection was aborted by the software in your host machine An unhandled exception occurred during the execut...
Bound UHMW DNA was resuspended in the elution buffer and quantified with Qubit dsDNA assay kits (ThermoFisher Scientific). DNA labeling was performed following the manufacturer’s protocols (Bionano Genomics). Standard Direct Labeling Enzyme 1 reactions were performed using 750 ng of purified UHMW...
Briefly, the DNA was eluted from the beads directly into an exonuclease digestion master mix (20 units of Plasmid-Safe ATP-dependent DNase (Epicentre), 1 mM ATP (Epicentre), 1× Plasmid-Safe Reaction Buffer (Epicentre)) in a 96-well plate. In a subset of samples, 1 μl of the ...
The PCR products were subjected to nucleic acid electrophoresis using 2% agarose gel with 0.5% TAE running buffer at 100 V for 40 min. We used DL500 DNA Marker (50–500 bp) (Cat# No. 3590A; TaKaRa, Osaka, Japan) to observe the bands under ultraviolet irradiation. Oligonucleotide ...
The first dimension was run in 5% polyacrylamide, the second dimension in 7.5% polyacrylamide, and both in TBE buffer with 8 M urea for 1.5 h at 200 V. 2D electrophoresis begins with an electrophoresis in the first dimension to separate the RNA molecules according to their mass. The...
as well as the volatility and unpredictability of the financial market in general, an adequate buffer should be provided to cope with the changes to the products and transactions brought about due to the market factors to prevent undue restriction on the future business operations of the Group. ...
Cytoplasmic/Nuclear fractionation Cells were resuspended in RLN buffer (50 mM Tris- HCl, pH 7.0, 140 mM NaCl, 1.5 mM M gCl2, 0.5% NP-40) and incubated on ice for 1 min. Then the cell lysates were centrifuged at 300 g for 3 min to collect the supernatants as ...