The refined 19-A X-ray crystal structure of D-Phe-Pro-Arg chloromethylketone-inhibited human alpha-thrombin: structure analysis, overall structure, electrostatic properties, detailed active-site geometry, and structure-function relations... Thrombin is a multifunctional serine proteinase that plays a ke...
Infrared spectra of chymotrypsinogen in 2H 2O have been measured as a function of pressure up to 30 kbar. An irreversible denaturation is induced at 7.6 kbar where the contributions of the random coil and turn conformational substructures to the protein structure increase dramatically at the ...
The extinction coefficient is certainly a function of the composition of the water phase, in the same way as tho Raman intensities for the water vibration band are a function of the solute concontration and type in aqueous soll.\tions4•6 • We thank Dr. W. A. Senior for his advice...
The observed rate coefficient (kobs) for aggregation displays a maximum as a function of temperature because of the competition between folding−unfolding thermodynamics and the intrinsic growth and nucleation rates; the latter contribution has a large, negative activation enthalpy that dominates kobs ...
Although this is a dominant theme, there is clearly heterogeneity, which we believe is a function of both the severity and/or repetition of the injury and the individual's immune response to (recurrent) pancreatic injury. Thus, in the North American Pancreatitis Study II...
The chapter presents an investigation concerning the C-terminal sequence by the technique of hydrogen-tritium replacement has been recently initiated.doi:10.1016/B978-0-12-211850-0.50006-9P. DesnuelleD. GratecosM. CharlesR. PeanaskyM. RoveryStructure–Function Relationships of Proteolytic Enzymes...
At all denaturant concentrations the enthalpy term slightly dominates the entropy term in the Gibbs free energy function. The most obvious effect of alkylureas was lowering of the temperature of transition, which was increasing with alkylurea concentration and the size of alkyl chain. Destabilization...
The spectral positions of the decay‐associated spectra of the hydrated powders do not shift as a function of hydration. This indicates that the structures of the zymogen and the active enzyme are unaffected by hydration. The lifetimes of α‐chymotrypsinogen A in phosphate buffer pH 7.4 are τ...
Two orthogonal approaches are introduced that yield separate nucleation and growth timescales: (i) combining unseeded monomer loss kinetics with static light scattering of the resulting aggregates or (ii) seeded monomer loss kinetics as a function of number concentration of seed. The size of the ...
The primary experimental results are the kinetics of monomer recovery and changes in aggregate structure, size, and morphology during urea-promoted dissociation as a function of initial aggregate size, overall protein concentration, urea concentration, and salt concentration. Biphasic kinetics are observed...