Chymosin B concentrations were measured in plasma samples from two groups of calves (Group 1: calves sampled from birth to 24 hours; Group 2: calves sampled from Day 1 to 21 after birth) and from one cow during the peri-partum period. Detection limit of the assay was 9.0 ng/ml. ...
Expression of a synthetic copy of the bovine chymosin gene in Aspergillus awamori from constitutive and pH-regulated promoters and secretion using two diff... A copy of the bovine chymosin gene (chy) with a codon usage optimized for its expression in Aspergillus awamori was constructed starting ...
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Chymosin (EC 3.4.23.4) and rennet, the latter containing about 85% chymosin and 15% pepsin, have been compared according to their coagulation properties wi... Ekstrand,B - 《Biochimica Et Biophysica Acta》 被引量: 64发表: 1980年 加载更多来源...
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Martín BlascoInst Nacl Tecnol IndJoaquín BozzoInst Nacl Tecnol IndMiguel Ángel GalvagnoInst Nacl Tecnol IndProtein Expression and PurificationProduction in stirred-tank bioreactor of recombinant bovine chymosin B by a highlevel expression transformant clone of Pichia pastoris. Diego Gabriel Noseda,...
Initially unevenly distributed salt in Gouda cheese is responsible for a limited extent of aggregation of β-casein; it allows a relatively rapid degradation of the monomeric form during the first weeks of ripening. A broader specificity of chymosin on the accessible part of α s1 -casein as we...
calf prochymosin B. After PCR amplification and sub- sequent purification, the calf prochymosin B amplicons were blunt-ended with Klenow fragment DNA poly- merase, phosphorylated with DNA kinase and then cloned into EcoRV-cut pBlueScript SK. The PBS-SK was then cut with NcoI liberating calf ...
The action of chymosin on the Phe23-Phe24 bond of bovine αs1-casein, in citrate buffer (pH 6.2) at 30°C, was followed by reversed-phase HPLC quantification of residual αs1-casein or fragment 24–199 after different time periods and at different substrate concentrations. This allowed dete...
The invention provides a method for preparing chymosin by mixed culture fermentation of bean dregs and waste material from fungal culture (WMFC). The method comprises the steps of: adding the WMFC into wet bean dregs, inoculating a tiny mucor strain for solid fermentation, and extracting the ...