We validated Chem-map for the BET inhibitor JQ1 in human leukemia K562 cells, as JQ1 interactions have been characterized using Click-Chem-seq in the same cell line5. In parallel, we mapped the genome-wide binding sites of the inhibitor’s main target BRD4 using a specific antibody and the...
Chromatin assembly in a yeast whole-cell extract. Focuses on a study which found that DNA ligase I plays a significant role in template repair during assembly in a yeast whole-cell extract. Development of ... Schultz,Michael,C.,... - 《Proceedings of the National Academy of Sciences of th...
The loop size in derivatives isolated using the most 'physiological' conditions was 86 kb; this remained unchanged throughout the cell cycle. This loop size is probably an average of a range of loops of between 5 and 200 kb. 展开 关键词:...
Anxiety-like behaviour and neuronal chromatin organization fluctuate across the oestrous cycle and differ between sexes.aTwo stages of the oestrous cycle, proestrus (Pro, purple; high oestrogen–low progesterone) and early dioestrus (Die, light pink; low oestrogen–high progesterone) in mice mimic f...
Activating mutations in the tyrosine kinase JAK2 cause myeloproliferative neoplasms, clonal blood stem cell disorders with a propensity for leukaemic transformation. LIF signalling through JAK-STAT enables ES cell self-renewal. Here we show that mouse ES cells carrying the human JAK2V617F mutation coul...
Enhancers, promoters and protein-coding genes have conserved epigenomic features, with adaptations to the organization of a proportion of genes in operon units. We further identified features specific to sex chromosomes, cell cycle modes, germline identity and dosage compensation, and unusual combinations...
We demonstrate that snATAC-seq is comparable to snRNA-seq in the assignment of cell identity and can further refine our understanding of functional heterogeneity in the nephron. The majority of differentially accessible chromatin regions are localized to promoters and a significant proportion are ...
Chromatin changes in cell transformation: progressive unfolding of the higher-order structure during the evolution of rat hepatocyte nodules. A differentia... Using differential scanning calorimetry and complementary ultrastructural observations, we have characterized the status of chromatin during the transform...
This diagram illustrates protein–DNA interactions in a cell that has undergone lysis. Protein Preparation Handbook Learn more about how to desalt, buffer exchange, concentrate, and/or remove contaminants from protein samples, immunoprecipitat...
The content of X-sex chromatin was studied in the buccal epithelial cells of pregnant women with Rh-negative blood. It was found that in women with a titer of Rh antibodies the amount of cells with X-sex chromatin decreases. A clinical-cytological correlation in the mother-placenta-fetus syst...