方法 以立体定位注射技术将腺相关病毒 AAV2/8-CaMKIIα-ChR2-mCherry 注射到 SD 雄性大鼠内侧前额叶,当病毒注射 3 ～4 周后,采用免疫荧光组织化学技术检测标记蛋白(mCherry)在谷氨酸能神经元(特异启动子 CaMKIIα)的表达情况;利用在体细胞外记录技术观测谷氨酸能神经元所表达的通道蛋白(ChR2)对不同光刺激频率的...
ChR2- mCherry expression in cholinergic MS/DBB projection neurons and light-induced IPSCs in CA1 pyramidal cells.Daniel A. NagodeAiHui TangMiranda A. KarsonMatthias KlugmannBradley E. Alger
Co-expression of ChR2-mCherry and NpHR-YFP-Kv2.1.Chaowen WuElena IvanovaYi ZhangZhuoHua Pan
We used a lentivirus that expresses a fusion protein between an enhanced version of ChR2 (H134R mutation) and the fluorescent reporter mCherry under the control of the PRSX8 artificial promoter (a Phox2-responsive element) to drive ChR2-mCherry expression selectively into Phox2b-positive RVLM...