3.2. Indirect interactions between cholesterol and proteins: the role of biophysical properties of the membrane 3.2.1. Membrane order and hydration Besides direct interactions, cholesterol can affect protein function through alterations in bulk membrane biophysical parameters, which had been among the most...
For cell-based studies, we grew both neurons and fibroblast cells on large-area monolayer graphene sheets. In neurons on graphene, we find increased cell membrane cholesterol and significant increases in the number, the release probability, and the turnover rate of synaptic vesicles—all of which...
Lipoic acid and ascorbic acid do not disturb the original membrane lateral organization in spite of being able to reduce sterol oxidation rate. Next, I examined whether superlattice is related to lipid rafts observed in cells and how cholesterol content would affect lipid raft properties and protein...
it is a primary component of the membrane that surrounds each cell, and it is the starting material or an intermediatecompoundfrom which the body synthesizesbileacids, steroid hormones, and vitamin D. Cholesterol circulates in the bloodstream and is synthesized by theliverand several other organs...
membrane composition. Moreover, the methodology of computing the permeability coefficients is well-established and tested. Despite this interest there are no dedicated works which are focused on computing permeabilities in normal and cancer cell membranes for common anti-tumor drugs. There are also ...
tweezers can mimic the formation of a protrusion, so we used this method to investigate the effects of modifying not only actin (with latrunculin A) but also microtubules (with nocodazole) and the plasma membrane itself (with methyl-β-cyclodextrin) on the Chinese hamster ovary cell membrane. ...
3, B–E). In contrast to the punctate staining of NHF, NPC1 fibroblasts exhibited a uniform distribution across the surface of the plasma membrane. In agreement with the isolated plasma membrane data, average intensity of PFO-D4 labeling per cell was 3.7-fold greater on NPC1 fibroblasts ...
Cholesterol is an essential structural component of animal cell membranes. It is required to maintain membrane structural integrity and fluidity. 胆固醇是动物细胞膜的重要结构成分。要求保持膜结构的完整性和流动性。 Cholesterol also serves as a precursor for the biosynthesis of several hormones, bile acid...
To further improve the therapeutic delivery efficiency of Lipo and better mimic the cell membrane composition, we proposed to engineer an improved lipid bilayer through covalently conjugating sphingomyelin (SM) that has a double aliphatic chain and is one of the core phospholipids in the cell ...
elegans seems to be involved in mTORC1 activation as the RNAi-induced knock-down has no effect on dauer exit in daf-2 (Fig. S5). It is therefore likely that an alternative molecular mechanism mediates cholesterol/mTORC1 signaling through the lysosomal membrane that has yet to be discovered. ...