CHO cell line with stable expression of the HTRA1 gene as a tool for studying functional activity of 5-HTreceptor ligandsIn order to obtain the appropriate CHO (Chinese Hamster Ovary) cell line overexpressing 5-hydroxytryptamine 1A receptor gene (HTR1A gene, human cDNA clone ref. ID NM_...
parental CHO-K1 glutamate synthase knockout (GS KO) host cell line. We conducted a thorough analysis of dynamic gene expression changes using high throughput RNA sequencing (RNASeq) and quantitative polymerase chain reaction (qPCR) methods. During fed-batch...
To truncate a telomere of hChr.21 in CHO-K1 cells, the CHO-K1 cells containing hChr.21 were transfected with two CRISPR/Cas9 vectors, AP001657T5 and T6, and with the linearised truncating vector, pBS-TEL/Dq HisDv2-Fcy; Fur. The transfected cells were expanded for 5days and then treat...
Ross S, Englesberg E (1993) The competence progression model in CHO-K1 cells: the relationship between protein kinase C and immediate early gene expression in the insulin mitogenic signal. Biochim Biophys Acta (BBA)-Molecular Cell Res 1177:307–317 CAS Google Scholar Tan JGL, Lee YY, Wang...
expression of adenylyl cyclase 2 leads to the functional rescue of human 5-HT6 receptor in a CHODUKX cell line - Nawoschik, Smith, et al. - 2007 () Citation Context ... c-Myc epitope tag were incorporated in the forward primer. The resulting PCR products were cloned into the Xba Ian...
At post-transfection with Cas9 and sgRNA expression vectors, the five tested sgRNAs generated detectable range of indels from 10.0% to 20.7% in the Mgat1 locus (Fig. 2b). Two sgRNAs, sgRNA1 and sgRNA5, were selected for targeted integration into the Mgat1 locus. CHO-S cells were ...
CHO-K1 cells expressing the dopamine receptor 2 (CHO D2L, pcDNA3.1 D2L) were a kind gift of Dr. J. A. Arias-Montaño (Cinvestav, Mexico) The construct for secreted ecto-domain of the canine β1 subunit (Secβ1) was a kind gift of Dr. D. M. Fambrough (Johns Hopkins University,...