Since the original CHO cell line was reported in 1956, many variations of the cell line have been created for various purposes. CHO-K1 was generated from a single clone of CHO cells in 1957, and CHO-DXB11 (also known as CHO-DUKX) was subsequently made through mutagenesis with ...
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a) Screening of medium composition for stable cell-line selection, evaluated in terms of fluorescent cell enrichment. CHO-K1 cells transfected with the CelloSelect system were cultured under the indicated conditions. Blue color represents the proportion of fluorescent cells at seeding. In panels a ...
The stable recombinant SH-87 cell line expressing anti-HER2 monoclonal antibody was generated from CHO K1 (ATCC # CCL-61) and cultured in protein-free media comprising of 50% HyQ PF-CHO and 50% CD CHO, supplemented with 1 g/L sodium bicarbonate, 8 mM L-glutamine, 0.05% Pluronic F-68...
In the study, CHO-K1 cells (Chinese hamster ovary cell line) were treated for 24 h with Cd (5 and 10 µM) and EGCG (0.5 and 1 µM) together or separately. Cell viability, ATP content, total ROS activity, mitochondrial membrane potential and apoptosis potential were determined. The ...
Upon re-transfection, the resulting subclones showed the same specific productivity as their respective parental cell line. To enrich for cells with potentially stable improved properties, the 1% highest producers were sorted three times, 2 days after transient transfection e...
The cell lines were generated by transfection of the suspension-variant derivatives CHO-K1 (CHOK1SV) with the glutamine synthetase (GS) expression vector pcB72.3 containing light chain (LC) and heavy chain (HC) cDNA, each driven by the hCMV-MIE promoter [60, 61]. Cells were maintained in...
The evaluation of each step of the stable cell line generation process is reported below. Stable pool generation. The stringency of selection in the generated single and double GS-KO CHO-K1 and S cells was analyzed from a bulk culture of cells after Adalumimab transfection. In this experiment...
Cell lines and cultivation regimes The establishment of genetically identical CHO cell lines producing IgG antibodies was described before (Schwaigerlehner et al. 2019). In this study, two genetically modified CHO-K1 cell line cultivars expressing two specific human antibodies, namely 2G12 and 353/11...
Cell line development TheCHO cell lineswere produced using our standardized platform process as described previously [12] . In brief, CHO-K1 cells devoid of theglutamine synthetase(GS) gene were transfected by electroporation with a single transfection vector encoding the genes for the protein-of-in...