Chain G in the structural model corresponds to Toc34 (labelled as G). Extended Data Fig. 5 The cryo-EM densities in the cytoplasmic region above the TOC complex and the structure of Ctap4. a, The cryo-EM map of TOC-TIC supercomplex filtered through the Gaussian filter. The contour ...
The feature shared by all members of the plastid family (proplastids, elaioplasts, leukoplasts, amyloplasts, chromoplasts, etioplasts, and chloroplasts) is a pair of outer membranes, known as the envelope. These membranes together provide a flexible boun
A highly efficient cell-free Escherichia coli system for the coupled transcription/translation of chloroplast DNA is described. Evidence for the synthesis of more than 20 discrete size classes of [35S]-methionine-labelled polypeptides has been obtained by dodecylsulphate/polyacrylamide gel electrophoresis ...
Therefore, we believe that the mechanism involved is a strong fitting and polar interaction between CABP and Rubisco catalytic sites that is independent of substrate concentration [8,9,20]. This is consistent with the use of 14C-labelled CABP-binding assays as the most accurate method to ...
a barrier. The requirement of this kind of transport originates from the compartmentalization of many biochemical activities. For example, the production of ATP in the chloroplast depends on the existence of a proton (H+) gradient that itself cannot be built without the existence of two ...
The bimolecular fluorescence complementation (BiFC) assay has emerged as one of the most popular methods for analysing protein–protein interactions (PPIs) in plant biology. This includes its increasing use as a tool for dissecting the molecular mechanis
For calculation of Ca2 þ and Mg2 þ dissociation constants, the ratio between the fluorescence of the fluorescently labelled protein before and after the ther- mophoretic movement was determined at increasing cation concentrations (Fig. 1a). An increase in Mg2 þ concentration did not change ...
The RNA fragments were 3′ labelled with pCp-biotin (NU-1706-BIO, Jena Biosciences GmbH) by the T4 RNA ligase 1 (M0204S, NEB). The labeled RNA fragment was diluted 1:1000 to 100 pM, heated at 95°C for 2 min and incubated with increasing concentrations of in vitro expressed LEFKO ...
The polymerases synthesise DNA from a template using four fluorescently-labelled nucleotides within the ZMWs and thus sequencing requires no prior amplification of the DNA template. The width of the ZMWs permits light to enter and excite the fluorophore that is being incorporated into the growing ...
Hybridizations of defined amounts of total cellular RNA to labelled probes of the 16S rDNA and of the rbcL gene allowed estimations of the variations of the corresponding cellular RNA pools. These variations are well correlated with the changes of the ptDNA cellular levels. These results show ...