1. 芯片法 目前常用的检测方法有毛细管电泳法(Capillary Electrophoresis, CE)和芯片法(Chip Assay)。其中,毛细管电泳分析是通过不同突 … cdmd.cnki.com.cn|基于26个网页 2. 染色质免疫共沉淀 染色质免疫共沉淀(CHIP assay) 准备工作: 预冷 PBS,RIPA Buffer,细胞刮子(用保鲜膜包好后,埋冰下) ,离心机 1....
ChIP Assay Kit即Chromatin Immunoprecipitation(ChIP)Assay Kit,也称染色质免疫沉淀检测试剂盒或ChIP检测试剂盒,用于通过免疫沉淀来沉淀和目标蛋白结合的染色质片段,最后通过PCR或Southern等方法来检测沉淀的染色质片段的试剂盒。通常用于检测特定的转录因子或组蛋白等基因组DNA结合蛋白是否和预期的特定基因组DNA序列在同一复...
3. 丰富的实验经验:专业的ChIP服务部门,实验人员从事ChIP实验长达5年以上,上千次成功ChIP实验经验,针对不同动植物组织有不同的前处理方式。 服务流程: 1、样品准备:培养细胞(若样品为组织,可先培养原代细胞);固定,或者直接活细胞送样。 2、细胞固定:此步骤目的为将结合在DNA上的蛋白用共价形式交联在所结合的位点...
ChIP Assay Kit即Chromatin Immunoprecipitation (ChIP) Assay Kit，也称染色质免疫沉淀检测试剂盒或ChIP检测试剂盒，用于通过免疫沉淀来沉淀和目标蛋白结合的染色质片段，最后通过PCR或Southern等方法来检测沉淀的染色质片段的试剂盒。通常用于检测特定的转录因子或组蛋白等基因组DNA结合蛋白是否和预期的特定基因组DNA序列在...
sequencing, and bioinformatics analysis. Through all the above steps, we completed the ChIP-seq analysis. With the clean data, we can provide comprehensive data analysis including GO analysis, KEGG pathway analysis, motif analysis, and visualization. Below show some results in the ChIP Assay project...
Chromatin immunoprecipitation (ChIP) assay is a powerful tool to study protein-DNA interaction and chromatin changes associated with gene expression. In the related article, we used ChIP assay to evaluate CCA1 binding to the CCA1, LHY, TOC1 and GI upstream region, as well as histone modificatio...
ChromatinImmunoprecipitation(ChIP)AssayKit Catalog#17-295 Lot#2135690 KitComponents ProteinAAgarose/SalmonSpermDNA,Catalog# 16-157C.Onevialcontaining1.5mLpackedbeads with600µgsonicatedsalmonspermDNA,1.5mgBSA andapproximately4.5mgrecombinantProteinA. ...
Our ChIP assay kits avoid the problems of traditional sonication protocols, which require subjecting chromatin to harsh, denaturing conditions. Learn more.
The chip includes electrodes on a substrate. The electrodes include a working electrode, a reference electrode, and a counter electrode. The reference electrode is constructed so as to not have an intrinsic potential. A self-assembly monolayer is positioned on the reference electrode. The self-...
系统标签: chromatin chip assay centrifuge incubate sonication EpigenomeNoE-protocol:ChromatinImmunoprecipitation(ChIP)Assayhttp://.epigenome-noe.net/researchtools/protocol.php?protid=10 1of614/03/200711:32 ChromatinImmunoprecipitation(ChIP)Assay(PROT11) AntigoneKouskouti&IreneKyrmizi InstituteofMolecularBiology...