1. 芯片法 目前常用的检测方法有毛细管电泳法(Capillary Electrophoresis, CE)和芯片法(Chip Assay)。其中,毛细管电泳分析是通过不同突 … cdmd.cnki.com.cn|基于26个网页 2. 染色质免疫共沉淀 染色质免疫共沉淀(CHIP assay) 准备工作: 预冷 PBS,RIPA Buffer,细胞刮子(用保鲜膜包好后,埋冰下) ,离心机 1....
ChIP Assay Kit即Chromatin Immunoprecipitation(ChIP)Assay Kit,也称染色质免疫沉淀检测试剂盒或ChIP检测试剂盒,用于通过免疫沉淀来沉淀和目标蛋白结合的染色质片段,最后通过PCR或Southern等方法来检测沉淀的染色质片段的试剂盒。通常用于检测特定的转录因子或组蛋白等基因组DNA结合蛋白是否和预期的特定基因组DNA序列在同一复...
Enrichment of DNA bound to the protein of interest is the goal for chromatin immunoprecipitation. DNA levels can be determined by agarose gel electrophoresis or more commonly by quantitative polymerase chain reaction (qPCR). Before the s...
Enrichment of DNA bound to the protein of interest is the goal for chromatin immunoprecipitation. DNA levels can be determined by agarose gel electrophoresis or more commonly by quantitative polymerase chain reaction (qPCR). Before the sp...
ChIP Assay Kit ChIP Assay Kit即Chromatin Immunoprecipitation(ChIP)Assay Kit,也称染色质免疫沉淀检测试剂盒或ChIP检测试剂盒,用于通过免疫沉淀来沉淀和目标蛋白结合的染色质片段,*通过PCR或Southern等方法来检测沉淀的染色质片段的试剂盒。通常用于检测特定的转录因子或组蛋白等基因组DNA结合蛋白是否和预期的特定基因组DN...
ChIP Assay Kit即Chromatin Immunoprecipitation (ChIP) Assay Kit，也称染色质免疫沉淀检测试剂盒或ChIP检测试剂盒，用于通过免疫沉淀来沉淀和目标蛋白结合的染色质片段，最后通过PCR或Southern等方法来检测沉淀的染色质片段的试剂盒。通常用于检测特定的转录因子或组蛋白等基因组DNA结合蛋白是否和预期的特定基因组DNA序列在...
碧云天即将上线ChIP实验新利器—BeyoChIP™Enzymatic ChIP Assay Kit (Protein A/G磁珠)(P2083),也称BeyoChIP™酶解法ChIP检测试剂盒(蛋白A/G磁珠),该试剂盒使用微球核酸酶酶解替代传统的超声法进行染色质片段化,与常规的使用超声将染色质打断成几百个碱基对长的片段相比,而微球核酸酶法可将绝大多数DNA片段化至...
BeyoChIP™ ChIP Assay Kit (Protein A/G磁珠)即BeyoChIP™ Chromatin Immunoprecipitation (ChIP) Assay Kit with Protein A/G Magnetic Beads,也称染色质免疫沉淀检测试剂盒或ChIP检测试剂盒(蛋白A/G磁珠),用于通过免疫沉淀来沉淀和目标蛋白结合的染色质片段,最后通过PCR或Southern等方法来检测沉淀的染色质片段的...
The amount of immunoprecipitate used in each assay was determined empirically such that an equal amount of ACTIN was amplified. All PCR reactions were done in 25μl, starting with 4 min at 940C, followed by 23-36 cycles (depending on the region being amplified) of 940C(15 s), 600C(...
染色质免疫共沉淀技术解析转录调控蛋白hac1在蛋白分泌调控中的功能 热度: 28820SingleOakDrive•Temecula,CA92590 TechnicalSupport:T:800437-7500•F:800437-7502 .millipore CertificateofAnalysis ChromatinImmunoprecipitation(ChIP)AssayKit Catalog#17-295