ChIP-Chip stands for Chromatin Immunoprecipitation and chip in the sense of DNA microarray. It is a technique to determine the genome-wide binding sites of a DNA-binding protein. While the basic principle is the same for all species, there are some differences in handling cells. This protocol...
Incubate for 10 minutes at room temperature with gentle agitation on a rocking platform. Remove the fixation solution by spinning down at 500xg at 4°C for 5 minutes. To quench the reaction, for cell count of 1-5 million, add 3mL of 0.65M glycine solution; for cell count of 5-10 mill...
Our groundbreaking Legion ColdFront Hyper sets a new standard for gaming PC thermal design. Tailored for gamers, this innovative thermal system features fans spinning inward, working in tandem to efficiently expel hot air from the rear. Gone is the issue of...
The supernatant was discarded and the cell pellet was rinsed with 1× PBS by spinning at 1800 rpm for 5 min. The chondrocytes obtained as cell pellets were suspended in H-DMEM containing FBS (10%), L-ascorbic acid (50 µg mL−1), dexamethasone (1 mM), TGFβ-2 (50 ...
TypeSpinning Production Line Applicable IndustriesManufacturing Plant, Retail Warranty1 YEAR After Warranty ServiceVideo technical support, Online support, Spare parts After-sales Service ProvidedFree spare parts, Online support PRODUCT FEATURES WERE USED FOR THE ROTOR OF THE SAURER...
This is easily accomplished in a centrifuge fitted with adaptors for spinning microtiter plates. Place a standard 96-well plate in the adaptor to hold the tubes in place. Be sure to balance the rotor (i.e. place a microtiter plate and tubes of appro- priate mass in the rotor's opposing...
(8.5) MAA EL13 and 200 nm of 950K PMMA A4 was spun, with a 5 min bake at 180 °C following the spinning of each layer. To eliminate charging effects during electron-beam writing, a 15 nm aluminum anticharging layer was deposited by electron-beam evaporation. Electron-beam ...
As much as I enjoy my Friday morning ritual, it’s Thursday nights that often get my creative wheels spinning. The reason I mainly prefer shopping on Fridays is so that I can have my fridge-freezer-pantry well stocked and ready to go for the weekend. Playdates, impromptu get-togethers ...
Referring to both FIGS. 8 and 9, there is shown the process of creating the wall structure 40. By well known methods such as spinning, a negatively photoimagable polyimide layer 39 is applied over the entire surface of the substrate 36 to cover the bonding pads 32 and 34, oxide layer ...
Congress must fund the CHIPS for America Act, which will invest $52 billion in domestic semiconductor capacity and capability.