In Excel, the formula to use is: =2^-(Delta Ct) Replace ‘Delta Ct‘ with your own delta Ct values. In the above example, we will get the following results: Note that the ‘Fold enrichment‘ for the negative antibody should always be ‘1‘. ChIP qPCR analysis Excel template For ...
简介: Inflammatory Response & Autoimmunity EpiTect ChIP qPCR Array 炎症反应和自身免疫ChIP qPCR芯片 提供商: SAbiosciences 服务名称: 炎症反应和自身免疫ChIP qPCR芯片 地区: 美国 “英拜为您实验加速”技术服务网址:http://www.yingbio.com/服务热线:400-696-6643、 18019265738邮箱:daihp@yingbio.com 、 hui...
DNA损伤信号通路ChIP qPCR芯片 DNA Repair EpiTect ChIP qPCR Array联系购买 配送服务: 运费￥0.00 数量: (库存 9999 件) 产品详细 简介: DNA Repair EpiTect ChIP qPCR Array DNA损伤信号通路ChIP qPCR芯片 提供商: SAbiosciences 服务名称: DNA损伤信号通路ChIP qPCR芯片 地区: 美国 “英拜为您实验加速”技术...
(IgG–included in kit, H3-K27Me3–Cat. no. 49-1014, H3-K9Ac–Cat. no. 49-1009) were used for each ChIP experiment. 1% of the sonicated chromatin was set aside as input control. Optimized qPCR primers were used to amplify the SAT2 locus (Cat...
DNA损伤信号通路ChIP qPCR芯片分析参与DNA损伤信号通路的84个关键基因的组蛋白修饰状态或组蛋白密码。组蛋白修饰调节染色质结构和与相关基因的转录活性。。每天暴露在环境因素(如活性氧物种,使甲基化剂、紫外线,和其他电离辐射),甚至正常的生理过程(如复制和重组)都会损DNA。DNA损伤是公认的细胞周期检查点,这样细胞可以...
(1)目标基区域转录因子结合/组蛋白修饰的验证:CHIP-qPCR (2)检测目标基因的mRNA表达水平:RT-qPCR (3)检测目标基因蛋白质表达水平:Western blot 02、转录因子或组蛋白修饰添加基因的干扰实验(非靶向),验证是否影响目标基因表达和细胞功能 (1)转录因子或组蛋白修饰添加基因的干扰:基因的突变/敲降/敲除/过表达、相关...
The Entire ChampionChIP System Protocol Can Be Completed in A Single Day. The ChampionChIP System includes a simplified high-performance One-Day ChIP Kit, ChIP-Grade Antibodies, real-time PCR primers, and a FREE Excel-based data analysis template. The figure depicts how all the components of ...
The EpiTect Chip qPCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease 细胞谱系ChIP qPCR芯片分析细胞分化48个关键基因的组蛋白修饰状态或组蛋白密码。组蛋白修饰调节染色质结构,调节基因活性和特定的细胞状态。在胚胎...
amplification reaction in real time. The fluorescent droplet counting software installed on PC will calculate and count the brightness of each droplet by playing video on the software interface, and then draw the brightness map of the droplet through the derived Excel table to get the linear ...
decorated with H3K4me1-K9me2/3 modifications. We corroborated these new bioinformatic findings with experimental data by qPCR assays using newly developed primate and hominid specific qPCR assays which complement similar research tools. Finally, we analysed mouse UHRF1 ChIP-seq data withRepEnToolsand...