材料：水稻（Oryza sativa L. sub.japonica cv. Nipponbare）的野生型（WT）种子、ZFP36过表达的ZFP36 OE种子和OsLEA5沉默的OsLEA5 RNAi种子。方法：1）蛋白互作实验：酵母双杂交实验（Yeast two-hybrid assay）、双分子荧光互补实验(BiFC )、免疫共沉淀实验（Co-IP）、GST pull-down实验。2）ChIP-Seq：测序平...
染色质免疫沉淀(Chip)检测试剂盒_.pdf,碧云天生物技术/Beyotime Biotechnology 订货热线:400-1683301 或 800-8283301 订货 e-mail :order@ 技术咨询:info@ 碧云天网站 微信公众号 网址: ChIP Assay Kit 产品编号 产品名称 包装 P2078 ChIP Assay Kit 22次 产品简介:
1)蛋白互作实验:酵母双杂交实验(Yeast two-hybrid assay)、双分子荧光互补实验(BiFC )、免疫共沉淀实验(Co-IP)、GST pull-down实验。 2)ChIP-Seq:测序平台为北京百迈客生物科技有限公司Illumina HiSeq,PE125。 3)本氏烟草GUS瞬时表达实验。 3研究结果 1)ZFP36互作蛋白的发现 利用酵母双杂交实验,以ZFP36全长...
1.Komar, Dorota N., et al. "Chromatin immunoprecipitation assay for the identification of Arabidopsis protein-DNA interactions in vivo." JoVE (Journal of Visualized Experiments) 107 (2016): e53422. 2.Gendrel, Anne-Valérie, et al. ...
To perform ChIP assay, proteins and DNA are in vivo-crosslinked by formaldehyde, chromatin is sonicated into small fragments, and an antibody is used to immunoprecipitate the protein-DNA complexes [1]. The DNA fragments are further purified and subjected to various analyses, for instance, PCR, ...
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Two microliters of ChIP eluate were used per reaction to control the success of the immunoprecipitations using the following real-time PCR primer combinations: H3K4me3 positive control locus TaqMan assay (probe with 5′ FAM and 3′ blackhole quencher 1) [RPL30-F CAAGGCAAAGCGAAATTGGT; RPL30-...
(ANG), 63 Holstein (HOL), 85 Brown Swiss (BRS), 96 Fleckvieh (Austrian dual purpose Simmental – FLK), 37 Brahman (BRA), 57 Gir (GIR), and 105 Nelore (NEL) individuals, which were genotyped for 777,962 SNPs with the Illumina BovineHD Genotyping BeadChip assay (HD) (Illumina Inc,...
(DNA Release Buffer) CP6 (Reverse Buffer) CP7 (Binding Buffer) CP8 (Elution Buffer) Protease Inhibitor Cocktail (100X) Normal Mouse IgG (1 mg/ml) Anti-Dimethyl H3-K9 (1 mg/ml) Proteinase K (10 mg/ml) 8-Well Assay Strips (with frame) 8-Well Strip Caps F-Spin Column F-Collection ...
The complete array information of the 50 K rice SNP chip is provided in Supplementary Table 1. In silico validation of the assay involved preliminary screening of the designed array file for each selected SNP, including their p-convert values generated using Affymetrix power tool (APT) Axio...