39) was prepared by annealing and extending the single-stranded DNA oligonucleotides Ma-PylT-F and Ma-PylT-R (2 mM; Supplementary Table 1) using OneTaq 2x Master Mix (NEB). The annealing and extension process was performed using the following protocol on a thermocycler (BioRad, C1000 ...
RNAase A (Ambion) and proteinase K (ThermoFisher) treatment, ChIP DNA was extracted using a Min-Elute purification kit (Qiagen). Sequencing libraries of ChIP DNA and input controls were generated using the NEBNext Ultra DNA Library Prep Kit for Illumina (NE Biolabs) ...
The RNA was prepared as mentioned above. A 1-µg sample of capped eGFP-mRNA was mixed with mRNA decapping enzyme reaction buffer (NEB; final concentration 1×) in a total volume of 19.7 µl. The mixture was irradiated (except for the control without irradiation). Then either 0.3...
A. in Machine Learning meets Quantum Physics, Lecture Notes in Physics (eds Schütt, K. T. et al.) (Springer, 2020). Behler, J. Atom-centered symmetry functions for constructing high-dimensional neural networks potentials. J. Chem. Phys. 134, 074106 (2011). Article PubMed CAS Google ...
Mutagenesis was carried out in two steps introducing one point mutation each, applying the Q5® Site-Directed Mutagenesis Kit (NEB, Germany). The four pairs of mutagenic primers (Supplementary Table 4) targeted one histidine at the active centre each (H91F and H251F for lAbPPO4_(HF)2, ...
Versions Notes Abstract This work presents the on-line coupled meteorology–chemistry transport model BOLCHEM, based on the hydrostatic meteorological BOLAM model, the gas chemistry module SAPRC90, and the aerosol dynamic module AERO3. It includes parameterizations to describe natural source emissions, ...