Most importantly, cross-links between two proteins can only be established when suitable side chains are present in the right distance and are accessible. Therefore, the characterization of protein-protein interactions by chemical cross-linking often requires empirical testing of a variety of different ...
did not result in any voltage-dependent sodium current (Fig.2b). Immunoblot analysis of co-expressed proteins also verified the presence of fully spliced Nav1.5 when XNav1.5RECwas co-expressed with both N and C constructs, although the relative abundance of fully spliced product was low compared...
Analysis of the bulk properties of the microspheres by confocal Raman mapping revealed the 3D distribution of the protein showing large voids within the pores. Protein was found to be adsorbed at the interface with the PLGA oil phase following deposition on evaporation of the solvent. Protein was...
Proteome-wide non-cleavable crosslink identification with MS Annika 3.0 reveals the structure of theC. elegansBox C/D complex Crosslinking mass spectrometry enables the structural analysis of proteins and protein complexes and serves as a powerful tool in protein-protein interaction studies, however, ...
The Research Collaboratory for Structural Bioinformatics (RCSB) Protein Data Bank (PDB) supports scientific research and education worldwide by providing access to annotated information about three-dimensional (3D) structures of macromolecules (e.g., nucleic acids, proteins), and associated small molecule...
Biopolymer sequences (e.g., DNA, RNA, proteins and polysaccharides) and chemical processes (e.g., a batch or continuous polymer synthesis run in a chemical plant) have close similarities from the modelling point of view. When a set of sequences or processes is characterized by multivariate ...
NMR is one of the most powerful tools to obtain structural information of proteins in atomic resolution. However, an analysis of protein three-dimensional structure from NMR spectra usually requires laborious chemical shift assignment. We developed a new method for predicting the protein structural ...
To facilitate the detection of-sulfinylated proteins, we demonstrate that a clickable, electrophilic diazene probe (DiaAlk) enables capture and site-centric proteomic analysis of this OxiPTM. Using this workflow, we revealed a striking difference between sulfenic acid modification (-sulfenylation) and ...
Chapter 9—Protein Detection and Analysis Chapter 10—Enzyme Substrates and Assays Chapter 11—Probes for Cytoskeletal Proteins Chapter 12—Probes for Organelles Chapter 13—Probes for Lipids and Membranes Chapter 14—Fluorescent Tracers of Cell Morphology and F...
The large-scale analysis of total cell lysates from fully solubilized Jurkat T cells identified known fatty-acylated proteins and many new candidates, including nuclear proteins and in particular histone H3 variants. We demonstrate that histones H3.1, H3.2, and H3.3 are modified with fatty acid ...