Net charge of -3-3 at pH 7 and with the amino acids arginine15 or arginine15-alanine17 in the binding region; serine protease inhibitorsdoi:US20030096752 A1The present invention relates to aprotinin variants having improved enzyme-inhibitory, immunological and pharmacokinetic properties and their ...
charge42,47, for example, the negatively charged wild-type yeast protein Sup35 which carries a net excess of six acidic amino acids (aspartic and glutamic acids) over the number of basic side chains (lysine and arginine) in the domain that controls its phase-segregation response (Supplementary...
A common feature of TTFL proteins, particularly those in the negative arm, is that the proteins are enriched for Intrinsically Disordered Regions (IDRs)5. IDRs are protein regions characterized by an inability to adopt a stable tertiary structure; instead, IDRs exist in a collection of interconver...
Cell penetrating decapeptide arginine-glycine (RG)5 and a pH-sensitive masking decapeptide histidine-glutamic acid (HE)5 were conjugated at the PEG free end to produce pH sensitive with peptides outside micelles (PHPO), while the pH sensitive with peptides inside micelles (PHPI) are the ...
The fact that histone tails are enriched in positively charged lysine (K) and arginine (R) (in the case of H2B, 43% K/R in the tail [15/35] versus 14% K/R in the histone fold domain [13/91]) makes this region a feasible target for negatively charged RNA. Third, the solubility...
Clustered pentaarginine-amide with 2.8 mM total charge shows higher affinity and capacity than Q Sepharose with 215 mM total charge. Thus, an organized rather than random distribution of charges may produce adsorbents with higher capacity and selectivity, especially for biomolecules with inherent ...
Figure 3. Whole-cell current traces of glycine receptor α1(250) mutants. All measurements were carried out at 21 °C–23 °C, pH 7.2, [Cl−]in= [Cl−]out= 146 mm, pH 7.2, −60 mV. Whole-cell current traces were obtained at saturating concentrations of glycine (500 µmfor...
Elution is supported either using a salt or pH gradient, though salt buffers are more commonly used due to difficulties in the reproducibility and controllability associated with pH gradients. Mass spectrometry (MS) has provided critical insights into understand...
9 Self-aggregation of the pre-BCR induced by electrostatic interaction between themselves has been proposed to explain the generation of this tonic signal. Mutation of seven evolutionarily conserved, positively charged arginine residues in the N-terminal portion of λ5 chain, a component of the ...
Localization of the N-methyl-D-aspartate type glutamate receptor (NMDAR) to dendritic spines is essential for excitatory synaptic transmission and plasticity. Rather than remaining trapped at synaptic sites, NMDA receptors undergo constant cycling into a