the mice were placed in the training chamber and the freezing behavior of each mouse was assessed in the presence of the CS or US. The results showed that the formation of contextual fear memory was impaired by PEP-2 but not SCR-2 (Figure 8J). Two days after ...
On the training day, mice were injected with 10 µm/kg peptide (i.p.) 1 h before the test, and transferred to a rectangular chamber capable of delivering foot-shocks (Med Associates). Three paired CS and US trials were carried out in the experiment. First, mice were allowed to ...
The 1uM assembled complex of R2TP-TTT (in 20 mM HEPES pH 8.0, 140mM NaCl, 0.5mM TCEP) was cross-linked with a 100-fold excess of isotopically labeled N-hydroxysuccinimide (NHS) ester disuccinimidyl suberate (DSS-H12/D12, Creative Molecules Inc., Canada), with respect to the protein con...