The article discusses changes made by Beebe Medical Center in Lewes, Delaware, after the arrest of pediatrician Earl Bradley, for child sexual abuse including the new chaperone policy, and the establishment of a multidisciplinar...
In most situations, a chaperone will be a trainedhealth professional, such as a healthcare assistant, who is familiar with the procedure or examination they are observing. While patients can sometimes find it helpful to have a family member present, you shouldnot rely on themto act as a chap...
Ric-8A protein was loaded onto a Source 15Q column and eluted with a 500 mM NaCl gradient at 180 mM NaCl and subsequently loaded on a GE Healthcare HiLoad 16/600 Superdex 200 column for size-exclusion chromatography in gel filtration buffer containing 50 mM Tris pH 8, 150 mM ...
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The samples were heated at 97 °C for 10 min and separated on 4–20% precast polyacrylamide gel (Bio-Rad) and blotted on a nitrocellulose (GE Healthcare) membrane. After blotting, the membranes were blocked using 5% skim milk prepared in 0.1% Tween/TBS for 1 h at RT. Thereafter they ...
GroEL was expressed at 34 °C from a plasmid in E. coli and purified by subsequent ion exchange chromatography on DE-52 cellulose and gel filtration on a Sephacryl S-300 (GE Healthcare) column. Firefly luciferase with C-terminal Myc and His6 tags (Luc-MycHis) was expressed at 30 °C ...
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All proteins were expressed inEscherichia colistrain BL21 (DE3) and included an N-terminal 6 × His tag. Purification buffers included 20–50 mM Tris or phosphate pH 8.0 and 10 mM β-mercaptoethanol. Chromatography resins were purchased from GE Healthcare Bio-Sciences (Marlborough, MA), ex...
After subsequent cleavage of the GST tag using thrombin, the GST and thrombin were removed using a GST column followed by a benzamidine FF column (GE Healthcare). Before use in heme-binding assays, the proteins were run over a Superdex 200 column (GE Healthcare) and assessed for purity by...
NudC154-274 was first exchanged to NMR-buffer without DTT using a PD10 buffer exchange column (GE Healthcare, Buckinghamshire, UK). IPSL (N-(1-oxyl-2,2,5,5-tetramethyl-3-pyrrolidinyl)iodoacetamide) was added to a 10 times molar excess to the NudC154-274 in non-reduced buffer and incub...