The pre-processing also included the removal of the sequence adaptors. Fragments with both surviving read pairs were then subjected to in silico normalization using Trinity93 (version: r20140717). Two de novo assemblies were generated using ABySS v1.394 and Trinity. Based on the number and ...
DNA Repair☆ Y. Polosina, in Reference Module in Biomedical Sciences, 2014 Consequences of DNA Damage Modification, mispairing, and loss of bases change the accuracy of DNA replication leading to base substitution and frameshift mutations. Bases can form eight possible mispairs; these lead to base...
sequence complexitymicro-deletionmicro-insertionindelmutagenesisThe vast majority of mutations causing human genetic disease are single base-pair substitutions and micro-deletions. However, a substantial proportion of the remainder involves the insertion of novel bases that serve to alter the reading frame ...
The technology is based on the use of complex mixtures of chromosome-specific DNA sequences linked to a fluorochrome to visualize specific chromosomes in metaphase spreads by fluorescence in situ hybridization (FISH) analysis. The use of such probes hybridized to specific chromosomal sequences was ...
4a, b). These SNPs are widely distributed across the genomes, with 25 and 204 SNPs located in the coding sequence and promoter regions (5k upstream and downstream of genes) across 23 and 147 genes for T. elliotii and P. monticolus, respectively (Supplementary Table 4). We then carried ...
Site-Directed Mutagenesis Overview In vitro site-directed mutagenesis is an invaluable technique for studying gene and protein structure-function relationships and for modifying vector sequences to facilitate cloning and expression strategies. Several approaches to this technique have been published, but ...
摘要: The interaction of the 6-O methylguanine with cytosine and thymine was studied using the SCF method in combination with a London type expression for dispersion energy. The structure of the complex formed with cytosine differs from that found previously with guanine itself....
Activity could not be fully recovered, and only a single turnover is observed. Data suggest a significant rate enhancement resulting from the presence of K42 for transimination and general base catalysis. 展开 关键词: Escherichia coli Salmonella typhimurium Schiff Bases Pyridoxal Phosphate Lysine DNA...
A ___ of a nucleotide in DNA results in a change in the codons in mRNA from the point of the deletion. DNA Mutations: Different mutations will have different effects on the codon sequence in messenger RNA. mutations can either be deletion ...
To prepare double-helical oligomers as template DNA for synthesis of U6–34 RNA and U6–34(Δ5) RNA, which lacks A7, A11, A25, A26, and G27 of U6–34, complementary deoxyoligonucleotides of the sequence, 5′-CCTAATACGACTCACTATAGGAACGATACAGAGTTCGCTCGTGAAGCGTTCCA-3′ (S1) and 5′-TG...