To significantly shorten this incubation period, in order to eventually adapt this technique for clinical use, we used the fluorescent-based intracellular dye CFSE to label responding cells in a 4-day MLR and fluorescent cell sorting to isolate the divided alloreactive cells that could then be ...
welabelthetargetcellbyCFSEandimitatethecytotoxiceffectofcytotoxickillercelltothetargetcell.,thendetectionontheFCMafterstainedwithPI.Inoculatethemousewiththevaccinewhichweresatisfactoryofcytotoxiceffectinvitro.Afteroptimizingtherequirementofthestainedoftargetcells,thestimulatingtimeofeffectorcell,actiontimebetweentargetcells...
Cutting Edge: Contribution of Lung-Resident T Cell Proliferation to the Overall Magnitude of the Antigen-Specific CD8 T Cell Response in the Lungs followin... To address this question, we developed a novel, in vivo, dual-label system using intranasal CFSE and BrdU administration to identify vir...
‹Cell Proliferation Using Flow Cytometry Improved CFSE Alternatives for Cell Proliferation Permanently label cells with Invitrogen CellTrace fluorescent stains without affecting morphology or physiology to trace gen...
that of unlabeled U87 MG cells after 72h, and CFSE had no effect on the proliferation of SH-SY5Y cells. Conclusion under normal culture conditions in vitro, CFSE with final concentration of 1μmol/L is an ideal condition for labeling SH-SY5Y cells. Under this condition, CFSE can label ...
CellTrace™ CFSE 细胞增殖试剂盒适用于体外和体内标记细胞,采用染料稀释法通过流式细胞分析追踪多代细胞。 •卓越的性能 — 明亮的单峰染色可实现多代细胞可视化 •信号稳定时间长 — 染色后可在细胞中保留数天 •无毒 — 染色不会对细胞健康产生不利影响 ...
Objective: To develope and optimize of a novel assay for assessment of cytotoxicity by multiparameter flow cytometry. Method:To ascertain the concentration and cultured times, firstly, we label the target cell by CFSE and imitate the cytotoxic effect of cytotoxic killer cell to the target cell.,...
CFSE标记结合流式细胞术在研究NK细胞分裂与杀伤功能中的应用范艳莹,吴长有*增殖和杀伤功能的方法。[摘要]目的建立并优化CFSE标记结合流式细胞术检测人NK细胞分裂、方法利用CFSE标记磁珠纯化的人NK细胞,加或不加IL-2进行培养。分别在第3、5、7天收集细胞,流式细胞术对NK细胞的分裂增殖进行检测;利用IL-2活化NK细胞...
Name:5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN,CAS:150347-59-4.Use:A Non-fluorescent cell permeant amine-reactive probe for long term tracing of cell..Buy CFSE.Molecular Fomula:C29H19O11,Molar Mass:557.461,Melting Point:152-154 °C(
Cell division can be measured as successive halving of the fluorescence intensity of CFSE. Cells labeled with CFSE may be fixed and permeabilized for analysis of intracellular targets using standard formaldehyde-containing fixatives and saponin-based permeabilization buffers, such as the Foxp3/...