4. A. M. Aravanis, M. Lee, R. D. Klausner, Next-generation sequencing of circulating tumor DNA for early cancer detection. Cell168, 571-574 (2017).5. J.-P. Issa, CpG island methylator phenotype in cancer. Nature Reviews Cancer4, 988-993 (2004).6. H. Schwarzenbach, D. S. Hoon,...
[12] Narayan, A. et al. Ultrasensitive measurement of hotspot mutations in tumour DNA in blood using error-suppressed multiplexed deep sequencing. Cancer Res. 72, 3492–3498 (2012). [13] Pel, J. et al. Nonlinear electrophoretic response...
② Schutsky EK, et al.Nondestructive, base-resolution sequencing of 5-hydroxymethylcytosine using a DNA deaminase. Nat Biotechnol. 2018 Oct 8. pii: nbt.4204. doi: 10.1038/nbt.4204. PubMed PMID: 30295673. ③ Gao Y, et al. Whole-genome bisulfite sequencing analysis of circulating tumour DNA...
为了进一步评估PFE的临床效用,Esfahani等人将具有肿瘤特异性表达基因的启动子作为生物标志物,在cfDNA中对这些标志物区域进行靶向深度测序,然后评估其PFE(EPIC-seq (epigenetic expression inference from cell-free DNA-sequencing))。该方法可以用于癌症检测、癌症分型和免疫治疗监测。基...
Using whole-genome next-generation sequencing (NGS), researchers at the Central European Institute of Technology at Masaryk University in the Czech Republic—in collaboration with the EMBL Genomics Core Facility, Heidelberg,...
1):3475. Published 2020 Jul 21. doi:10.1038/s41467-020-17316-z 【7】Blauwkamp TA, Thair S, Rosen MJ, et al. Analytical and clinical validation of a microbial cell-free DNA sequencing test for infectious disease. Nat Microbiol. 2019;4(4):663-674. doi:10.1038/s41564-018-0349-6 ...
近日，来自美国诺华生物医学研究所的O. Alejandro Balbin团队在Science Translational Medicine杂志上发表了一篇题为 Clonal hematopoiesis detection in patients with cancer using cell-free DNA sequencing 的文章，他们提出了一种准确的分类方法，能够在没有匹配的WBC测序的情况下区分实体瘤患者血浆cfDNA中的血液衍生突变...
from non-small cell lung cancer patients. Cancer Res1999;59:67–70. 核小体在TSS区是否占据,影响cfDNA的深度,因此可以用来推测基因的表达情况 Ulz P, Thallinger G G, Auer M, et al. Inferring expressed genes by whole-genome sequencing of plasma DNA.[J]. Nature Genetics, 2016, 48(10):1273...
[1] Blauwkamp, T. A., Thair, S., Rosen, M. J., Blair, L., Lindner, M. S., Vilfan, I. D., ... & Cheung, A. (2019). Analytical and clinical validation of a microbial cell-free DNA sequencing test...