② Schutsky EK, et al.Nondestructive, base-resolution sequencing of 5-hydroxymethylcytosine using a DNA deaminase. Nat Biotechnol. 2018 Oct 8. pii: nbt.4204. doi: 10.1038/nbt.4204. PubMed PMID: 30295673. ③ Gao Y, et al. Whole-genome bisulfite sequencing analysis of circulating tumour DNA...
为了进一步评估PFE的临床效用,Esfahani等人将具有肿瘤特异性表达基因的启动子作为生物标志物,在cfDNA中对这些标志物区域进行靶向深度测序,然后评估其PFE(EPIC-seq (epigenetic expression inference from cell-free DNA-sequencing))。该方法可以用于癌症检测、癌症分型和免疫治疗监测。基...
2022年ctDNA高通量测序临床实践专家共识指出:随着液态活检的快速发展,采集ll体液对患者的分子特征进行分析成为可能,特别是基于ctDNA的高通量测序(next generation sequencing,NGS)技术,因其无创或微创、检测时间短、能够反映瘤内和转移灶异质性、可动态监测治疗疗效等优势而在临床得到越来越广泛的应用。 ctDNA 水平一般呈...
但这两种方法在很大程度上受到小样本量的限制,并且无法利用来自cfDNA的全谱表观遗传学信息。 美国加利福尼亚大学欧文分校医学院Wei Li团队与Helio Genomics公司首席科学官David J. Taggart等人合作,在Genome Medicine上发表了题为“Multimodal epigenetic sequencing analysis (MESA) of cell-free DNA for non-invasive c...
近日，来自美国诺华生物医学研究所的O. Alejandro Balbin团队在Science Translational Medicine杂志上发表了一篇题为 Clonal hematopoiesis detection in patients with cancer using cell-free DNA sequencing 的文章，他们提出了一种准确的分类方法，能够在没有匹配的WBC测序的情况下区分实体瘤患者血浆cfDNA中的血液衍生突变...
4. A. M. Aravanis, M. Lee, R. D. Klausner, Next-generation sequencing of circulating tumor DNA for early cancer detection. Cell168, 571-574 (2017).5. J.-P. Issa, CpG island methylator phenotype in cancer. Nature Reviews Cancer4, 988-993 (2004).6. H. Schwarzenbach, D. S. Hoon,...
1):3475. Published 2020 Jul 21. doi:10.1038/s41467-020-17316-z 【7】Blauwkamp TA, Thair S, Rosen MJ, et al. Analytical and clinical validation of a microbial cell-free DNA sequencing test for infectious disease. Nat Microbiol. 2019;4(4):663-674. doi:10.1038/s41564-018-0349-6 ...
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SequencingCell-free DNA (cfDNA) is acquiring increasingly importance in oncologic clinical practice, mostly due to its role in predicting the onset of therapy resistance by following the mutation status...doi:10.1007/978-1-4939-8973-7_9Michela Tebaldi...
ONT另一主要优势在于可获取长读长序列，而捕获更长cfDNA片段有可能发现新的生物标志物。文章发表在Genome Biology近日，来自以色列希伯来大学等单位的研究人员在Genome Biology杂志发表了题为“Detecting cell‑of‑origin and cancer‑specific methylation features of cell‑free DNA from Nanopore sequencing”的研究...